Institute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, People's Republic of China.
Institute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, People's Republic of China.
Int J Biol Macromol. 2024 Jul;273(Pt 1):133032. doi: 10.1016/j.ijbiomac.2024.133032. Epub 2024 Jun 9.
Collagen's unique properties promise hemostatic potential, but its sponge form's stability and mechanics need improvement. In this study, we developed a series of homeostatic sponges by co-assembling collagen and curdlan at different ratios into hydrogels, followed by freeze-drying treatment. The incorporation of curdlan into collagen sponges has been found to significantly enhance the sponge's properties, including increased porosity, elevated water uptake, improved elasticity, and enhanced resistance to degradation. In vitro cytotoxicity and hemolysis assays have demonstrated the biocompatibility and nontoxicity of composite sponges. In mouse liver perforation and incision models, the composite sponges achieved rapid coagulation within 67 s and 75 s, respectively, outperforming gauze and gelatin sponge in reducing blood loss. Furthermore, composite sponges demonstrated superior wound healing potential in mice full-thickness skin defects model, with accelerated healing rates observed at days 3, 7, and 14 compared to the control group. Overall, collagen/curdlan composite sponge show promise for hemostasis and wound healing applications.
胶原蛋白独特的性质使其具有止血的潜力,但它的海绵形式的稳定性和力学性能仍需要改进。在本研究中,我们通过将不同比例的胶原蛋白和琼脂糖共组装成水凝胶,然后进行冷冻干燥处理,开发了一系列的自稳海绵。研究发现,琼脂糖的掺入可显著增强海绵的性能,包括增加孔隙率、提高吸水性、改善弹性和增强抗降解能力。体外细胞毒性和溶血实验表明复合海绵具有良好的生物相容性和无毒特性。在小鼠肝穿孔和切口模型中,复合海绵分别在 67s 和 75s 内实现了快速凝血,其在减少失血量方面优于纱布和明胶海绵。此外,在小鼠全层皮肤缺损模型中,复合海绵表现出更好的伤口愈合潜力,与对照组相比,在第 3、7 和 14 天观察到愈合速度加快。总的来说,胶原蛋白/琼脂糖复合海绵在止血和伤口愈合应用方面具有广阔的前景。
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