Frontier Institute of Science and Technology, and State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
Frontier Institute of Science and Technology, and State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China; Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an 710049, China.
J Colloid Interface Sci. 2019 Nov 15;556:514-528. doi: 10.1016/j.jcis.2019.08.083. Epub 2019 Aug 24.
Infection is a major obstacle to wound healing. To enhance the healing of infected wounds, dressings with antibacterial activities and multifunctional properties to promote wound healing are highly desirable. Herein, gelatin-grafted-dopamine (GT-DA) and polydopamine-coated carbon nanotubes (CNT-PDA) were used to engineer antibacterial, adhesive, antioxidant and conductive GT-DA/chitosan/CNT composite hydrogels through the oxidative coupling of catechol groups using a HO/HRP (horseradish peroxidase) catalytic system. The addition of the antibiotic doxycycline endowed the hydrogels with antimicrobial activity to treat infected full-thickness defect wounds. Additionally, CNT-PDA endowed these hydrogels with an excellent photothermal effect, leading to good in vitro and in vivo antibacterial activities against Gram-positive and Gram-negative bacteria. The catechol group and polydopamine imparted tissue adhesiveness, and the hemostatic and antioxidant abilities of these hydrogels were also investigated. The porosity, degradability, swelling, rheological, mechanical, and conductive behaviors of these hydrogels were finely regulated by changing the concentration of CNT-PDA. Hemolysis and cytocompatibility tests using L929 fibroblast cells confirmed the good biocompatibility of these hydrogels. The wound closure, collagen deposition, histomorphological examination and immunofluorescence staining results demonstrated the excellent effects of these hydrogels in an infected full-thickness mouse skin defect wound. In summary, the adhesive antibacterial and conductive GT-DA/chitosan/CNT hydrogels showed great potential as multifunctional bioactive dressings for the treatment of infected wounds.
感染是伤口愈合的主要障碍。为了增强感染伤口的愈合能力，人们非常希望使用具有抗菌活性和多功能特性的敷料来促进伤口愈合。在此，通过使用 HO/HRP（辣根过氧化物酶）催化体系氧化偶联儿茶酚基团，使用接枝有多巴胺的明胶（GT-DA）和聚多巴胺涂覆的碳纳米管（CNT-PDA）来构建具有抗菌、粘附、抗氧化和导电性能的抗菌、粘附、抗氧化和导电 GT-DA/壳聚糖/CNT 复合水凝胶。添加抗生素强力霉素赋予水凝胶抗菌活性，以治疗感染的全层缺陷伤口。此外，CNT-PDA 赋予这些水凝胶优异的光热效应，导致对革兰氏阳性和革兰氏阴性细菌具有良好的体外和体内抗菌活性。儿茶酚基团和聚多巴胺赋予组织粘附性，还研究了这些水凝胶的止血和抗氧化能力。通过改变 CNT-PDA 的浓度，可以精细调节这些水凝胶的孔隙率、降解性、溶胀性、流变学、机械性能和导电性。使用 L929 成纤维细胞进行溶血和细胞相容性测试证实了这些水凝胶具有良好的生物相容性。伤口闭合、胶原蛋白沉积、组织形态学检查和免疫荧光染色结果表明，这些水凝胶在感染的全层小鼠皮肤缺陷伤口中具有出色的效果。总之，具有粘附性的抗菌和导电 GT-DA/壳聚糖/CNT 水凝胶作为治疗感染伤口的多功能生物活性敷料具有巨大的潜力。
