Department of Biochemistry, Government College University, Faisalabad, 38000, Pakistan.
Faculty of Pharmacy, Hamdard University Islamabad Campus, Islamabad, Pakistan.
Inflammopharmacology. 2024 Jun;32(3):1929-1940. doi: 10.1007/s10787-024-01450-x. Epub 2024 Mar 31.
Gout is a metabolic condition characterized by the accumulation of urate crystals in the synovial joints. These crystal depositions result in joint swelling and increased concentration of serum uric acid in blood. The commercially available drugs lower serum uric acid levels and reduce inflammation, but these standard therapies have many side effects. This study aimed to investigate anti-gout and anti-inflammatory properties of curcumin nanoparticles (CNPs). For this purpose, CNPs were prepared by dissolving curcumin into dichloromethane. Then, gout was induced by injecting monosodium urate crystals (MSU) in the ankle joint and in the intra-peritoneal cavity which caused ankle swelling and increased blood uric acid levels. CNPs in different concentrations (5, 10, and 20 ppm) and allopurinol were orally administered. The MSU crystals increased the xanthine oxidase levels both in serum and the liver. Moreover, MSU crystals increased the serum levels of interleukin 1β, interleukin-6, tumor necrosis factor-alpha, liver function tests markers, renal function tests markers, and lipid profiles. However, the administration of CNPs decreased the levels of all these variables. CNPs increased the serum high-density lipoprotein and interleukin-10 levels. Moreover, CNPs also reduced ankle swelling significantly. Hence, the levels of xanthine oxidase, uric acid and ankle swelling were reduced significantly by oral administration of CNPs. Our findings indicate that CNPs through their anti-inflammatory properties significantly alleviate gouty arthritis. Thus, the study concluded that CNPs can be developed as an efficient anti-gout agent with minimal side effects.
痛风是一种代谢性疾病，其特征是尿酸盐晶体在滑膜关节中积累。这些晶体沉积物导致关节肿胀和血液中血清尿酸浓度增加。市售的药物可以降低血清尿酸水平并减轻炎症，但这些标准疗法有许多副作用。本研究旨在研究姜黄素纳米粒子（CNPs）的抗痛风和抗炎特性。为此，将姜黄素溶解在二氯甲烷中制备 CNPs。然后，通过向踝关节和腹腔内注射单钠尿酸盐晶体（MSU）诱导痛风，导致踝关节肿胀和血液尿酸水平升高。以不同浓度（5、10 和 20 ppm）和别嘌呤醇口服给予 CNPs。MSU 晶体增加了血清和肝脏中的黄嘌呤氧化酶水平。此外，MSU 晶体增加了血清白细胞介素 1β、白细胞介素-6、肿瘤坏死因子-α、肝功能试验标志物、肾功能试验标志物和脂质谱的水平。然而，CNPs 的给药降低了所有这些变量的水平。CNPs 增加了血清高密度脂蛋白和白细胞介素 10 的水平。此外，CNPs 还显著减轻了踝关节肿胀。因此，通过口服 CNPs，黄嘌呤氧化酶、尿酸和踝关节肿胀的水平显著降低。我们的研究结果表明，CNPs 通过其抗炎特性显著缓解痛风性关节炎。因此，研究得出结论，CNPs 可以开发为具有最小副作用的有效抗痛风药物。
