Holland J P, Holland M J
J Biol Chem. 1979 Oct 10;254(19):9839-45.
The complete nucleotide sequence of the coding, as well as the flanking noncoding regions, of a yeast glyceraldehyde-3-phosphate dehydrogenase gene was determined. Both the 5' and 3' noncoding sequences are extremely AT-rich and regions of partial dyad symmetry are present immediately adjacent to the 5' and 3' ends of the translated portion of the gene. The sequence AAUAAA is present in the 3' noncoding region of this gene and is a part of an extensive region of dyad symmetry which is structurally related to the 3'-terminal portion of both procaryotic mRNAs, as well as some eukaryotic mRNAs. The coding region of this gene does not contain intervening sequences. Establishment of the primary structure of this glyceraldehyde-3-phosphate dehydrogenase gene provides a basis for further studies involving in vitro mutation of the gene and subsequent analysis of gene expression in vivo.
测定了酵母甘油醛-3-磷酸脱氢酶基因编码区以及侧翼非编码区的完整核苷酸序列。5'和3'非编码序列都富含AT，并且在基因翻译部分的5'和3'末端紧邻处存在部分二元对称区域。序列AAUAAA存在于该基因的3'非编码区，是二元对称广泛区域的一部分，该区域在结构上与原核mRNA以及一些真核mRNA的3'末端部分相关。该基因的编码区不包含间隔序列。该甘油醛-3-磷酸脱氢酶基因一级结构的确定为进一步研究该基因的体外突变及随后体内基因表达分析提供了基础。
