Fujii T, Kobayashi O, Yoshimoto H, Furukawa S, Tamai Y
Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., Kanagawa, Japan.
Appl Environ Microbiol. 1997 Mar;63(3):910-5. doi: 10.1128/aem.63.3.910-915.1997.
The reduction of acetate ester synthesis by aeration and the addition of unsaturated fatty acids to the medium has been reported to be the result of the reduction in alcohol acetyltransferase (AATase) activity induced by inhibition of this enzyme. However, regulation of the AATase gene ATF1 has not been reported. In this study, ATF1 gene expression was studied by Northern analysis, and the results showed that the ATF1 gene was repressed both by aeration and by unsaturated fatty acids. The results also showed that the reduction of AATase activity is closely related to the degree of repression of ATF1 mRNA, which suggested that the gene repression is the primary means of reducing AATase activity in vivo. Using the Escherichia coli lacZ gene as a reporter gene, it was shown that a 150-bp fragment of the 5' flanking sequence played a major role in the repression by aeration and unsaturated fatty acid addition.
据报道，通气以及向培养基中添加不饱和脂肪酸会导致醋酸酯合成减少，这是由于抑制该酶导致酒精乙酰转移酶（AATase）活性降低的结果。然而，尚未有关于AATase基因ATF1调控的报道。在本研究中，通过Northern分析研究了ATF1基因的表达，结果表明，通气和不饱和脂肪酸均可抑制ATF1基因。结果还表明，AATase活性的降低与ATF1 mRNA的抑制程度密切相关，这表明基因抑制是体内降低AATase活性的主要方式。使用大肠杆菌lacZ基因作为报告基因，结果表明5'侧翼序列的150 bp片段在通气和添加不饱和脂肪酸引起的抑制中起主要作用。
