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基于免疫基因的预后模型，构建了用于预测原发性肝癌相关免疫基因和免疫细胞浸润情况的预后模型及其实验验证。

Based on the prognosis model of immunogenes, the prognosis model was constructed to predict the invasion of immune genes and immune cells related to primary liver cancer and its experimental validation.

作者信息

Yang Yu-Ping, Bai Min, Cheng Yin-Xia, Feng Xin, Zhang Yan-Ying, Zhang Yuan-Yuan, Liu Meng-Ya, Duan Yong-Qiang

机构信息

Gansu University of Traditional Chinese Medicine, College of Basic Medical Sciences, Lanzhou, 730000, PR China.

Ningxia Medical University, College of Traditional Chinese Medicine, Yinchuan, 750000, PR China.

出版信息

Heliyon. 2024 Mar 16;10(7):e27362. doi: 10.1016/j.heliyon.2024.e27362. eCollection 2024 Apr 15.

DOI:10.1016/j.heliyon.2024.e27362
PMID:38560168
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10980948/
Abstract

BACKGROUND

Primary liver cancer (PLC) is a prevalent malignancy of the digestive system characterized by insidious symptom onset and a generally poor prognosis. Recent studies have highlighted a significant correlation between the initiation and prognosis of liver cancer and the immune function of PLC patients.

PURPOSE

Revealing the expression of PLC-related immune genes and the characteristics of immune cell infiltration provides assistance for the analysis of clinical pathological parameters and prognosis of PLC patients.

METHODS

PLC-related differentially expressed genes (DEGs) with a median absolute deviation (MAD > 0.5) were identified from TCGA and GEO databases. These DEGs were intersected with immune-related genes (IRGs) from the ImmPort database to obtain PLC-related IRGs. The method of constructing a prognostic model through immune-related gene pairs (IRGPs) is used to obtain IRGPs and conduct the selection of central immune genes. The central immune genes obtained from the selection of IRGPs are validated in PLC. Subsequently, the relative proportions of 22 types of immune cells in different immune risk groups are evaluated, and the differential characteristics of PLC-related immune cells are verified through animal experiments.

RESULTS

Through database screening and the construction of an IRGP prognosis model, 84 pairs of IRGPs ( < 0.001) were ultimately obtained. Analysis of these 84 IRGPs revealed 11 central immune genes related to PLC, showing differential expression in liver cancer tissues compared to normal liver tissues. Results from the CiberSort platform indicate differential expression of immune cells such as naive B cells, macrophages, and neutrophils in different immune risk groups. Animal experiments demonstrated altered immune cell proportions in H22 tumor-bearing mice, validating findings from peripheral blood and spleen homogenate analyses.

CONCLUSION

Our study successfully predicted and validated PLC-related IRGs and immune cells, suggesting their potential as prognostic indicators and therapeutic targets for PLC.

摘要

背景

原发性肝癌（PLC）是消化系统常见的恶性肿瘤，其症状隐匿，预后通常较差。最近的研究强调了肝癌的发生和预后与PLC患者免疫功能之间的显著相关性。

目的

揭示PLC相关免疫基因的表达及免疫细胞浸润特征，为分析PLC患者的临床病理参数和预后提供帮助。

方法

从TCGA和GEO数据库中鉴定出中位数绝对偏差（MAD>0.5）的PLC相关差异表达基因（DEG）。将这些DEG与ImmPort数据库中的免疫相关基因（IRG）进行交叉分析，以获得PLC相关的IRG。采用通过免疫相关基因对（IRGP）构建预后模型的方法来获得IRGP并进行核心免疫基因的筛选。从IRGP筛选中获得的核心免疫基因在PLC中进行验证。随后，评估不同免疫风险组中22种免疫细胞的相对比例，并通过动物实验验证PLC相关免疫细胞的差异特征。

结果

通过数据库筛选和构建IRGP预后模型，最终获得84对IRGP（<0.001）。对这84对IRGP的分析揭示了11个与PLC相关的核心免疫基因，与正常肝组织相比，这些基因在肝癌组织中表现出差异表达。CiberSort平台的结果表明，不同免疫风险组中幼稚B细胞、巨噬细胞和中性粒细胞等免疫细胞存在差异表达。动物实验表明，荷H22肿瘤小鼠的免疫细胞比例发生了变化，验证了外周血和脾匀浆分析的结果。

结论

我们的研究成功预测并验证了PLC相关的IRG和免疫细胞，表明它们作为PLC预后指标和治疗靶点的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f97/10980948/dba2ed48a6a9/gr7a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f97/10980948/22ab9ca5bd1f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f97/10980948/b00b63350343/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f97/10980948/e275dbceaed2/gr3a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f97/10980948/fd287d8cc438/gr4a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f97/10980948/c2ae68378bbd/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f97/10980948/0f68b46704d5/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f97/10980948/dba2ed48a6a9/gr7a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f97/10980948/22ab9ca5bd1f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f97/10980948/b00b63350343/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f97/10980948/e275dbceaed2/gr3a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f97/10980948/fd287d8cc438/gr4a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f97/10980948/c2ae68378bbd/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f97/10980948/0f68b46704d5/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f97/10980948/dba2ed48a6a9/gr7a.jpg

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本文引用的文献

1
NDRG1 in Aggressive Breast Cancer Progression and Brain Metastasis.NDRG1 在侵袭性乳腺癌进展和脑转移中的作用。
J Natl Cancer Inst. 2022 Apr 11;114(4):579-591. doi: 10.1093/jnci/djab222.
2
Cancer-cell stiffening via cholesterol depletion enhances adoptive T-cell immunotherapy.胆固醇耗竭导致癌细胞变硬，从而增强过继性 T 细胞免疫疗法。
Nat Biomed Eng. 2021 Dec;5(12):1411-1425. doi: 10.1038/s41551-021-00826-6. Epub 2021 Dec 6.
3
Neutrophils in cancer carcinogenesis and metastasis.中性粒细胞在癌症发生和转移中的作用。
J Hematol Oncol. 2021 Oct 21;14(1):173. doi: 10.1186/s13045-021-01187-y.
4
Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and Epithelial-Mesenchymal Transition Process in Gastric Cancer.钙结合蛋白S100A16促进胃癌的增殖、侵袭及上皮-间质转化过程
Front Cell Dev Biol. 2021 Sep 28;9:736929. doi: 10.3389/fcell.2021.736929. eCollection 2021.
5
Emerging role of PD-L1 modification in cancer immunotherapy.PD-L1修饰在癌症免疫治疗中的新作用。
Am J Cancer Res. 2021 Aug 15;11(8):3832-3840. eCollection 2021.
6
Computational analysis for identification of early diagnostic biomarkers and prognostic biomarkers of liver cancer based on GEO and TCGA databases and studies on pathways and biological functions affecting the survival time of liver cancer.基于 GEO 和 TCGA 数据库的肝癌早期诊断生物标志物和预后生物标志物的计算分析，以及影响肝癌患者生存时间的通路和生物学功能研究。
BMC Cancer. 2021 Jul 8;21(1):791. doi: 10.1186/s12885-021-08520-1.
7
Circular RNA hsa_circ_0110389 promotes gastric cancer progression through upregulating SORT1 via sponging miR-127-5p and miR-136-5p.环状 RNA hsa_circ_0110389 通过海绵吸附 miR-127-5p 和 miR-136-5p 而上调 SORT1 促进胃癌进展。
Cell Death Dis. 2021 Jun 23;12(7):639. doi: 10.1038/s41419-021-03903-5.
8
Tumor Immune Microenvironment and Immunosuppressive Therapy in Hepatocellular Carcinoma: A Review.肝癌肿瘤免疫微环境与免疫抑制治疗：综述。
Int J Mol Sci. 2021 May 28;22(11):5801. doi: 10.3390/ijms22115801.
9
The immune-related biomarker TEK inhibits the development of clear cell renal cell carcinoma (ccRCC) by regulating AKT phosphorylation.免疫相关生物标志物TEK通过调节AKT磷酸化来抑制肾透明细胞癌（ccRCC）的发展。
Cancer Cell Int. 2021 Feb 18;21(1):119. doi: 10.1186/s12935-021-01830-1.
10
Development and verification of an immune-related gene pairs prognostic signature in ovarian cancer.卵巢癌免疫相关基因对预后signature 的构建与验证。
J Cell Mol Med. 2021 Mar;25(6):2918-2930. doi: 10.1111/jcmm.16327. Epub 2021 Feb 4.