Li Liubing, Wang Min, Chen Qi, Zhang Mingxing, Chen Zhihao, Han Mingxiao, Zhao Chenhao, Xie Zonggang, Dong Qirong, Zhang Haifang
Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, China.
Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, China.
Heliyon. 2024 Mar 21;10(7):e28461. doi: 10.1016/j.heliyon.2024.e28461. eCollection 2024 Apr 15.
() has the ability to invade human cortical bones and cause intracellular infections in osteoblasts, which may lead to a long-term infection that is difficult to eliminate. It is critical to identify the underlying mechanisms of the osteoblast response to the intracellular . More recently, multiple circular RNA (circRNA) functions have been identified, including serving as protein scaffolds or miRNA sponges and being translated into polypeptides. The role that circRNAs play in intracellular infection of osteoblasts has not, to our knowledge, been investigated. Here, we established an intracellular infection model of in osteoblasts and compared the circRNA expression of osteoblasts between the infected and control groups using RNA sequencing technology, by which a significant difference was found. In total, 117 upregulated and 125 down-regulated differentially expressed circRNAs (DEcircRNAs) were identified, and reverse transcription-quantitative PCR was employed to validate the results of RNA sequencing. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses demonstrated that DEcircRNAs were enriched in processes associated with macromolecule modification, cellular component organization or biogenesis, and intracellular non-membrane-bound organelles. Finally, a potentially important network of circRNA-miRNA-mRNA based on the DEcircRNAs was constructed. Overall, this study revealed the circRNA expression profile of human osteoblasts infected by intracellular for the first time, and identified the circRNAs that may contribute to the pathogenesis of infectious diseases caused by intracellular infection in human osteoblasts.
（）能够侵入人类皮质骨并在成骨细胞中引起细胞内感染，这可能导致难以消除的长期感染。确定成骨细胞对细胞内（）反应的潜在机制至关重要。最近，已确定了多种环状RNA（circRNA）的功能，包括作为蛋白质支架或微小RNA（miRNA）海绵以及被翻译成多肽。据我们所知，circRNA在成骨细胞细胞内（）感染中所起的作用尚未得到研究。在此，我们建立了成骨细胞内（）感染模型，并使用RNA测序技术比较了感染组和对照组中成骨细胞的circRNA表达，由此发现了显著差异。总共鉴定出117个上调和125个下调的差异表达环状RNA（DEcircRNA），并采用逆转录定量PCR验证RNA测序结果。基因本体论和京都基因与基因组百科全书通路分析表明，DEcircRNA在与大分子修饰、细胞成分组织或生物发生以及细胞内无膜结合细胞器相关的过程中富集。最后，基于DEcircRNA构建了一个潜在的重要circRNA-miRNA-mRNA网络。总体而言，本研究首次揭示了细胞内（）感染的人类成骨细胞的circRNA表达谱，并鉴定出可能导致人类成骨细胞内（）感染引起的传染病发病机制的circRNA。
