Hwang Ji Hye
Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Gachon University, Seongnam, Republic of Korea.
J Pharmacopuncture. 2024 Mar 31;27(1):38-46. doi: 10.3831/KPI.2024.27.1.38.
Genotoxicity is evaluated through a chromosomal aberration test using cultured mammalian cells to determine the toxicity of no-pain pharmacopuncture (NPP), which has recently been used to treat musculoskeletal pain disorders in Korean medical clinical practice.
An initial test was performed to determine the dosage range of the NPP, followed by the main test. In this study, NPP doses of 10.0, 5.0, and 2.5%, and negative and positive controls were tested. An chromosome aberration test was performed using Chinese hamster lung cells under short-term treatment with or without metabolic activation and under continuous treatment without metabolic activation.
Compared with the saline negative control group, NPP did not significantly increase the frequency of chromosomal abnormalities in Chinese hamster lung cells, regardless of the presence or absence of metabolic activation. Additionally, the number of cells with structural chromosomal abnormalities was significantly higher in the positive control group than that in the negative control group that received saline.
Based on the above results, the chromosomal abnormality-producing effect of NPP was determined to be negative under these test conditions.
通过使用培养的哺乳动物细胞进行染色体畸变试验来评估基因毒性，以确定无痛药针疗法（NPP）的毒性，该疗法最近在韩国医学临床实践中用于治疗肌肉骨骼疼痛疾病。
先进行初步试验以确定NPP的剂量范围，然后进行主要试验。在本研究中，测试了10.0%、5.0%和2.5%的NPP剂量以及阴性和阳性对照。使用中国仓鼠肺细胞在有或无代谢活化的短期处理以及无代谢活化的连续处理下进行染色体畸变试验。
与生理盐水阴性对照组相比，无论有无代谢活化，NPP均未显著增加中国仓鼠肺细胞中染色体异常的频率。此外，阳性对照组中具有结构染色体异常的细胞数量显著高于接受生理盐水的阴性对照组。
基于上述结果，在这些试验条件下，NPP产生染色体异常的作用被确定为阴性。
