Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA.
Department of Horticulture and Crop Sciences, The Ohio State University, Columbus, Ohio 43210, USA.
Anal Methods. 2024 Apr 25;16(16):2449-2455. doi: 10.1039/d4ay00082j.
Carotenoids are yellow, orange, and red pigments commonly found in plants. In leaves, these molecules are essential for photosynthesis, but they also play a major role in plant growth and development. Efficiently monitoring concentrations of specific carotenoids in plant tissues could help to explain plant responses to environmental stressors, infection and disease, fertilization, and other conditions. Previously, Raman methods have been used to demonstrate a correlation between plant fitness and the carotenoid content of leaves. Due to solvatochromatic effects and structural similarities within the carotenoid family, current Raman spectroscopy techniques struggle to assign signals to specific carotenoids with certainty, complicating the determination of amounts of individual carotenoids present in a sample. In this work, we use thin layer chromatography-Raman spectroscopy, or TLC-Raman, to identify and quantify carotenoids extracted from tomato leaves. These quick and accurate methods could be applied to study the relationship between pigment content and a number of factors affecting plant health.
类胡萝卜素是黄色、橙色和红色的色素，在植物中普遍存在。在叶子中，这些分子对光合作用至关重要，但它们在植物生长和发育中也起着重要作用。有效地监测植物组织中特定类胡萝卜素的浓度可以帮助解释植物对环境胁迫、感染和疾病、受精和其他条件的反应。以前，拉曼方法已经被用于证明植物适应性与叶子中叶黄素含量之间的相关性。由于溶剂化效应和类胡萝卜素家族内的结构相似性，当前的拉曼光谱技术难以确定信号与特定类胡萝卜素的确切对应关系，从而使确定样品中存在的单个类胡萝卜素的量变得复杂。在这项工作中，我们使用薄层色谱-拉曼光谱法（TLC-Raman）来鉴定和定量从番茄叶片中提取的类胡萝卜素。这些快速准确的方法可以应用于研究色素含量与影响植物健康的许多因素之间的关系。
