Department of Orthopedics and Traumatology, Medical Faculty, Duzce University, Düzce, Turkey.
Department of Pathology, Medical Faculty, Duzce University, Düzce, Turkey.
J Orthop Res. 2024 Sep;42(9):1907-1915. doi: 10.1002/jor.25844. Epub 2024 Apr 2.
Although the Achilles tendon is the largest and strongest tendon in the body, healing of the Achilles tendon is the most common injury, and this process is difficult due to poor tendon circulation; moreover, the underlying mechanism has not been fully elucidated. In our study, we aimed to investigate the effects of pentoxifylline and alpha-tocopherol administered separately or in combination on rats with Achilles tendon injury. Forty-eight male Wistar rats weighing 230 ± 30 g were used in the study. The rats were randomly divided into eight groups of six animals each. Tendons were evaluated histopathologically and biomechanically. According to the statistical analysis, the vascularity density in the pentoxifylline group on day 14 was significantly greater than that in the other groups (p < 0.05). The collagen arrangement in the pentoxifylline and alpha-tocopherol groups on day 14 was found to be firmer and smoother than that in the control group (p < 0.05). The collagen arrangement in the pentoxifylline group on day 28 was greater than that in the other groups (p < 0.05). The biomechanical results were significantly greater in all groups (p < 0.05). Pentoxifylline contributed to tendon healing both through neovascularization in the early period and by improving collagen orientation in the late period, while alpha-tocopherol had a positive effect on collagen orientation in the early period. No beneficial effects were observed when pentoxifylline and alpha-tocopherol were used together. We believe that further research is needed to understand the effects of this combination therapy on tendon healing.
尽管跟腱是人体中最大最强壮的肌腱，但它却是最常受伤的部位，而且由于其血液循环较差，其愈合过程较为困难；此外，其潜在机制尚未完全阐明。在我们的研究中，我们旨在研究己酮可可碱和α-生育酚单独或联合给药对跟腱损伤大鼠的影响。我们使用了 48 只雄性 Wistar 大鼠，体重为 230±30g，将其用于本研究。这些大鼠被随机分为 8 组，每组 6 只。对肌腱进行组织病理学和生物力学评估。根据统计分析，己酮可可碱组在第 14 天的血管密度明显大于其他组（p<0.05）。在第 14 天，己酮可可碱和α-生育酚组的胶原排列比对照组更坚固和更光滑（p<0.05）。在第 28 天，己酮可可碱组的胶原排列大于其他组（p<0.05）。所有组的生物力学结果均显著增大（p<0.05）。己酮可可碱通过早期的新生血管形成和晚期改善胶原取向对肌腱愈合都有促进作用，而α-生育酚对早期的胶原取向有积极作用。当己酮可可碱和α-生育酚联合使用时，没有观察到有益的效果。我们认为，需要进一步研究来了解这种联合治疗对肌腱愈合的影响。
