MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, P. R. China.
Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, South China Normal University, Guangzhou, 510631, P. R. China.
Small Methods. 2024 Nov;8(11):e2400122. doi: 10.1002/smtd.202400122. Epub 2024 Apr 2.
Near-infrared fluorescence (NIRF)/photoacoustic (PA) dual-modality imaging integrated high-sensitivity fluorescence imaging with deep-penetration PA imaging has been recognized as a reliable tool for disease detection and diagnosis. However, it remains an immense challenge for a molecule probe to achieve the optimal NIRF and PA imaging by adjusting the energy allocation between radiative transition and nonradiative transition. Herein, a simple but effective strategy is reported to engineer a NIRF/PA dual-modality probe (Cl-HDN) based on the near-infrared hemicyanine scaffold to optimize the energy allocation between radiative and nonradiative transition. Upon activation by HS, the Cl-HDN shows a 3.6-fold enhancement in the PA signal and a 4.3-fold enhancement in the fluorescence signal. To achieve the sensitive and selective detection of HS in vivo, the Cl-HDN is encapsulated within an amphiphilic lipid (DSPE-PEG) to form the Cl-HDN-LP, which can successfully map the changes of HS in a tumor-bearing mouse model with the NIRF/PA dual-modality imaging. This work presents a promising strategy for optimizing fluorescence and PA effects in a molecule probe, which may be extended to the NIRF/PA dual-modality imaging of other disease-relevant biomarkers.
近红外荧光 (NIRF)/光声 (PA) 双模成像是一种将高灵敏度荧光成像与深度穿透 PA 成像相结合的可靠方法，已被认为是用于疾病检测和诊断的工具。然而，通过调整辐射跃迁和非辐射跃迁之间的能量分配，使分子探针达到最佳的 NIRF 和 PA 成像仍然是一个巨大的挑战。在此，报道了一种简单但有效的策略，基于近红外半花菁骨架构建 NIRF/PA 双模探针 (Cl-HDN)，以优化辐射和非辐射跃迁之间的能量分配。在 HS 的激活下，Cl-HDN 的 PA 信号增强了 3.6 倍，荧光信号增强了 4.3 倍。为了实现 HS 的灵敏和选择性体内检测，将 Cl-HDN 包封在两亲性脂质 (DSPE-PEG) 中形成 Cl-HDN-LP，可成功地对荷瘤小鼠模型中的 HS 变化进行 NIRF/PA 双模成像。这项工作提出了一种优化分子探针中荧光和 PA 效应的有前途的策略，该策略可能扩展到其他与疾病相关的生物标志物的 NIRF/PA 双模成像。
