Marshall Laboratory of Biomedical Engineering, International Cancer Center, Laboratory of Evolutionary Theranostics (LET), School of Biomedical Engineering , Shenzhen University Health Science Center , Shenzhen 518060 , P. R. China.
Anal Chem. 2019 Jun 4;91(11):7112-7117. doi: 10.1021/acs.analchem.9b00109. Epub 2019 May 22.
Alkaline phosphatase (ALP) is distributed widely in living organisms and is an important biomarker closely related to many physiological and pathological processes. However, in vivo real-time detection of ALP remains a significant challenge. Herein, we developed a turn-on molecular probe (denoted as LET-3) to visualize ALP activity in tumor tissues through near-infrared fluorescence (NIRF) and photoacoustic (PA) dual-modal imaging. LET-3, composed of NIR hemicyanine dye (LET-CyOH) and a phosphate moiety, showed a 23-fold NIRF enhancement at 730 nm and 27-fold PA enhancement at 710 nm upon activation by ALP. More importantly, both in vitro and in vivo diagnostic experiments indicated that LET-3 has a high sensitivity and good selectivity for ALP. These findings provide a promising strategy for in vivo ALP detection using NIRF and PA dual-channel turn-on probes.
碱性磷酸酶（ALP）广泛分布于生物体内，是一种与许多生理和病理过程密切相关的重要生物标志物。然而，在体实时检测 ALP 仍然是一个重大挑战。在此，我们开发了一种开启型分子探针（命名为 LET-3），通过近红外荧光（NIRF）和光声（PA）双模态成像来可视化肿瘤组织中的 ALP 活性。LET-3 由近红外半花菁染料（LET-CyOH）和磷酸部分组成，在被 ALP 激活后，在 730nm 处的近红外荧光增强 23 倍，在 710nm 处的光声增强 27 倍。更重要的是，体外和体内诊断实验表明，LET-3 对 ALP 具有高灵敏度和良好的选择性。这些发现为使用 NIRF 和 PA 双通道开启型探针进行体内 ALP 检测提供了一种有前景的策略。
