National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang, 550025, China.
National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang, 550025, China.
Talanta. 2024 Jul 1;274:126004. doi: 10.1016/j.talanta.2024.126004. Epub 2024 Apr 1.
Reactive oxygen species (ROS), reactive nitrogen species (RNS), and reactive sulfur species (RSS) serve as vital mediators essential for preserving intracellular redox homeostasis within the human body, thereby possessing significant implications across physiological and pathological domains. Nevertheless, deviations from normal levels of ROS, RNS, and RSS disturb redox homeostasis, leading to detrimental consequences that compromise bodily integrity. This disruption is closely linked to the onset of various human diseases, thereby posing a substantial threat to human health and survival. Small-molecule fluorescent probes exhibit considerable potential as analytical instruments for the monitoring of ROS, RNS, and RSS due to their exceptional sensitivity and selectivity, operational simplicity, non-invasiveness, localization capabilities, and ability to facilitate in situ optical signal generation for real-time dynamic analyte monitoring. Due to their distinctive transition from their spirocyclic form (non-fluorescent) to their ring-opened form (fluorescent), along with their exceptional light stability, broad wavelength range, high fluorescence quantum yield, and high extinction coefficient, rhodamine fluorophores have been extensively employed in the development of fluorescent probes. This review primarily concentrates on the investigation of fluorescent probes utilizing rhodamine dyes for ROS, RNS, and RSS detection from the perspective of different response groups since 2016. The scope of this review encompasses the design of probe structures, elucidation of response mechanisms, and exploration of biological applications.
活性氧（ROS）、活性氮（RNS）和活性硫（RSS）作为重要的介质，对于维持人体细胞内的氧化还原平衡至关重要，在生理和病理领域都具有重要意义。然而，ROS、RNS 和 RSS 的水平异常会打破氧化还原平衡，导致有害后果，危及身体完整性。这种破坏与各种人类疾病的发生密切相关，对人类健康和生存构成重大威胁。小分子荧光探针由于其出色的灵敏度和选择性、操作简单性、非侵入性、定位能力以及促进实时动态分析物监测的原位光学信号生成的能力，在 ROS、RNS 和 RSS 的监测方面具有很大的潜力，是一种分析仪器。由于其独特的螺环形式（非荧光）到开环形式（荧光）的转变，以及出色的光稳定性、宽波长范围、高荧光量子产率和高消光系数，罗丹明荧光染料已被广泛用于荧光探针的开发。本综述主要集中于研究 2016 年以来基于不同响应基团的用于 ROS、RNS 和 RSS 检测的罗丹明染料荧光探针，包括探针结构的设计、响应机制的阐明和生物应用的探索。
