State Key Laboratory of Freshwater Ecology and Biotechnology, and Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China.
School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province, 266109, China.
Poult Sci. 2024 Jun;103(6):103673. doi: 10.1016/j.psj.2024.103673. Epub 2024 Mar 16.
Type IV interferon (IFN) has been shown to be a cytokine with antiviral activity in fish and amphibian. But, it has not been cloned and characterized functionally in avian species. In this study, type IV IFN, IFN-υ, and its 2 possible receptors, IFN-υR1 and IL10RB, were identified from an avian species, the mallard (Anas platyrhynchos). Mallard IFN-υ has a 531 bp open reading frame (ORF), encoding 176 amino acids (aa), and has highly conserved features as reported in different species, with an N-terminal signal peptide and a predicted multi-helix structure. The IFN-υR1 and IL10RB contain 528 and 343 aa, respectively, with IFN-υR1 protein containing JAK1 and STAT binding sites, and IL10RB containing TYK2 binding site. These 2 receptor subunits also possess 3 domains, the N-terminal extracellular domain, the transmembrane domain, and the C-terminal intracellular domain. Expression analysis indicated that IFN-υ, IFN-υR1 and IL10RB were widely expressed in examined organs/tissues, with the highest level observed in pancreas, blood, and kidney, respectively. The expression of IFN-υ, IFN-υR1 and IL10RB in liver, spleen or kidney was significantly upregulated after stimulation with polyI:C. Furthermore, recombinant IFN-υ protein induced the expression of ISGs, and the receptor of IFN-υ was verified as IFN-υR1 and IL10RB using a chimeric receptor approach in HEK293 cells. Taken together, these results indicate that IFN-υ is involved in the host innate immune response in mallard.
IV 型干扰素（IFN）已被证明在鱼类和两栖类动物中具有抗病毒活性的细胞因子。但是，它尚未在禽类中被克隆和功能表征。在这项研究中，从禽类鸭（Anas platyrhynchos）中鉴定出了 IV 型 IFN、IFN-υ及其 2 种可能的受体 IFN-υR1 和 IL10RB。鸭 IFN-υ 具有 531bp 的开放阅读框（ORF），编码 176 个氨基酸（aa），并具有不同物种报道的高度保守特征，具有 N 端信号肽和预测的多螺旋结构。IFN-υR1 和 IL10RB 分别包含 528 和 343 个 aa，IFN-υR1 蛋白含有 JAK1 和 STAT 结合位点，IL10RB 含有 TYK2 结合位点。这 2 个受体亚基还具有 3 个结构域，即 N 端细胞外结构域、跨膜结构域和 C 端细胞内结构域。表达分析表明，IFN-υ、IFN-υR1 和 IL10RB 在检查的器官/组织中广泛表达，胰腺、血液和肾脏中的表达水平最高。polyI:C 刺激后，肝、脾或肾中的 IFN-υ、IFN-υR1 和 IL10RB 的表达显著上调。此外，重组 IFN-υ 蛋白诱导 ISGs 的表达，并使用 HEK293 细胞中的嵌合受体方法验证 IFN-υ 的受体为 IFN-υR1 和 IL10RB。总之，这些结果表明 IFN-υ 参与了鸭的宿主固有免疫反应。
