Lv Wei, Shi Wenmin, Zhang Zhijuan, Ru Lihua, Feng Weisheng, Tang Hanxiao, Wang Xiangqi
College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China.
College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China; Institute of Mass Spectrometer and Atmospheric Environment, Jinan University, Guangzhou, 510632, China.
Anal Biochem. 2024 Jul;690:115527. doi: 10.1016/j.ab.2024.115527. Epub 2024 Mar 31.
The identification of noninvasive volatile biomarkers for lung cancer is a significant clinical challenge. Through in vitro studies, the recognition of altered metabolism in cell volatile organic compound (VOC) emitting profile, along with the occurrence of oncogenesis, provides insight into the biochemical pathways involved in the production and metabolism of lung cancer volatile biomarkers. In this research, for the first time, a comprehensive comparative analysis of the volatile metabolites in NSCLS cells (A549), SCLC cells (H446), lung normal cells (BEAS-2B), as well as metabolites in both the oxidative stress (OS) group and control group. Specifically, the combination of eleven VOCs, including n-dodecane, acetaldehyde, isopropylbenzene, p-ethyltoluene and cis-1,3-dichloropropene, exhibited potential as volatile biomarkers for lung cancer originating from two different histological sources. Furthermore, the screening process in A549 cell lines resulted in the identification of three exclusive biomarkers, isopropylbenzene, formaldehyde and bromoform. Similarly, the exclusive biomarkers 1,2,4-trimethylbenzene, p-ethyltoluene, and cis-1,3-dichloropropene were present in the H446 cell line. Additionally, significant changes in trans-2-pentene, acetaldehyde, 1,2,4-trimethylbenzene, and bromoform were observed, indicating a strong association with OS. These findings highlight the potential of volatile biomarkers profiling as a means of noninvasive identification for lung cancer diagnosis.
肺癌非侵入性挥发性生物标志物的识别是一项重大的临床挑战。通过体外研究，识别细胞挥发性有机化合物（VOC）排放谱中代谢的改变以及肿瘤发生的情况，有助于深入了解肺癌挥发性生物标志物产生和代谢所涉及的生化途径。在本研究中，首次对非小细胞肺癌细胞（A549）、小细胞肺癌细胞（H446）、肺正常细胞（BEAS-2B）中的挥发性代谢物，以及氧化应激（OS）组和对照组中的代谢物进行了全面的比较分析。具体而言，包括正十二烷、乙醛、异丙苯、对乙基甲苯和顺式1,3-二氯丙烯在内的11种VOC的组合，展现出作为源自两种不同组织学来源肺癌的挥发性生物标志物的潜力。此外，在A549细胞系中的筛选过程鉴定出了三种独特的生物标志物，即异丙苯、甲醛和溴仿。同样，独特的生物标志物1,2,4-三甲基苯、对乙基甲苯和顺式1,3-二氯丙烯存在于H446细胞系中。此外，还观察到反式-2-戊烯、乙醛、1,2,4-三甲基苯和溴仿有显著变化，表明与氧化应激有密切关联。这些发现突出了挥发性生物标志物谱作为肺癌诊断非侵入性识别手段的潜力。
