鳄嘴花甲醇提取物及其馏分的体外抗氧化和α-葡萄糖苷酶抑制活性以及综合代谢产物分析
In vitro antioxidant and, α-glucosidase inhibitory activities and comprehensive metabolite profiling of methanol extract and its fractions from Clinacanthus nutans.
作者信息
Alam Md Ariful, Zaidul I S M, Ghafoor Kashif, Sahena F, Hakim M A, Rafii M Y, Abir H M, Bostanudin M F, Perumal V, Khatib A
机构信息
Faculty of Pharmacy, International Islamic University Malaysia (IIUM), Kuantan Campus, 25200, Kuantan, Pahang, Malaysia.
Department of Food Science and Nutrition, King Saud University, Riyadh, 11451, Saudi Arabia.
出版信息
BMC Complement Altern Med. 2017 Mar 31;17(1):181. doi: 10.1186/s12906-017-1684-5.
BACKGROUND
This study was aimed to evaluate antioxidant and α-glucosidase inhibitory activity, with a subsequent analysis of total phenolic and total flavonoid content of methanol extract and its derived fractions from Clinacanthus nutans accompanied by comprehensive phytochemical profiling.
METHODS
Liquid-liquid partition chromatography was used to separate methanolic extract to get hexane, ethyl acetate, butanol and residual aqueous fractions. The total antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging and ferric reducing antioxidant power assay (FRAP). The antidiabetic activity of methanol extract and its consequent fractions were examined by α-glucosidase inhibitory bioassay. The chemical profiling was carried out by gas chromatography coupled with quadrupole time-of-flight mass spectrometry (GC Q-TOF MS).
RESULTS
The total yield for methanol extraction was (12.63 ± 0.98) % (w/w) and highest fractionated value found for residual aqueous (52.25 ± 1.01) % (w/w) as compared to the other fractions. Significant DPPH free radical scavenging activity was found for methanolic extract (63.07 ± 0.11) % and (79.98 ± 0.31) % for ethyl acetate fraction among all the fractions evaluated. Methanol extract was the most prominent in case of FRAP (141.89 ± 0.87 μg AAE/g) whereas most effective reducing power observed in ethyl acetate fraction (133.6 ± 0.2987 μg AAE/g). The results also indicated a substantial α-glucosidase inhibitory activity for butanol fraction (72.16 ± 1.0) % and ethyl acetate fraction (70.76 ± 0.49) %. The statistical analysis revealed that total phenolic and total flavonoid content of the samples had the significant (p < 0.05) impact on DPPH free radical scavenging and α-glucosidase inhibitory activity.
CONCLUSION
Current results proposed the therapeutic potential of Clinacanthus nutans, especially ethyl acetate and butanol fraction as chemotherapeutic agent against oxidative related cellular damages and control the postprandial hyperglycemia. The phytochemical investigation showed the existence of active constituents in Clinacanthus nutans extract and fractions.
背景
本研究旨在评估抗氧化和α-葡萄糖苷酶抑制活性，并随后分析风车草甲醇提取物及其衍生馏分中的总酚和总黄酮含量，同时进行全面的植物化学分析。
方法
采用液-液分配色谱法分离甲醇提取物，得到己烷、乙酸乙酯、丁醇和残余水相馏分。通过2,2-二苯基-1-苦基肼自由基清除法（DPPH）和铁还原抗氧化能力测定法（FRAP）测定总抗氧化活性。通过α-葡萄糖苷酶抑制生物测定法检测甲醇提取物及其后续馏分的抗糖尿病活性。通过气相色谱-四极杆飞行时间质谱联用（GC Q-TOF MS）进行化学分析。
结果
甲醇提取物的总产率为（12.63±0.98）%（w/w），与其他馏分相比，残余水相的分馏值最高，为（52.25±1.01）%（w/w）。在所有评估的馏分中，甲醇提取物的DPPH自由基清除活性显著，为（63.07±0.11）%，乙酸乙酯馏分为（79.98±0.31）%。在FRAP方面，甲醇提取物最为突出（141.89±0.87μg AAE/g），而乙酸乙酯馏分的还原能力最强（133.6±0.2987μg AAE/g）。结果还表明，丁醇馏分（72.16±1.0）%和乙酸乙酯馏分（70.76±0.49）%具有显著的α-葡萄糖苷酶抑制活性。统计分析表明，样品中的总酚和总黄酮含量对DPPH自由基清除和α-葡萄糖苷酶抑制活性有显著（p<0.05）影响。
结论
目前的结果表明风车草具有治疗潜力，尤其是乙酸乙酯和丁醇馏分，可作为抗氧化相关细胞损伤的化疗药物并控制餐后高血糖。植物化学研究表明风车草提取物和馏分中存在活性成分。
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