Alabi Gbenga Oluwaseyi, Elekofehinti Olusola Olalekan, Sanni David Morakinyo, Ashaolu Joseph Opeolu, Oluwatuyi Adedotun Olayemi
Bioinformatics and Molecular Biology Unit, Department of Biochemistry, Federal University of Technology Akure, Ondo State, Nigeria.
Teady Bioscience Research Laboratory, 42, Adinlewa Street, Akure, Ondo State, Nigeria.
Toxicol Rep. 2024 Oct 22;13:101774. doi: 10.1016/j.toxrep.2024.101774. eCollection 2024 Dec.
Prostate cancer (Pca) is a deadly disease prevalent among men, and it accounts for about 7-8 % of mortality globally. Synthetic drugs have proved effective but have limitations and severe side effects. There is, therefore, a need to discover a less expensive, natural therapeutic agent with no side effects in treating the ailment.
The study aims to investigate the anti-prostate cancer activity of extracts of () at the physiological and molecular levels in experimental animals.
Polyphenol-rich extract of was subjected to HPLC analysis to identify the plant's phytochemical constituent. Adult Wistar rats were orally administered 2mls of 50, 100 and 200 PPM of the cacodylic acid solution for 28 days to induce prostate cancer, while treatment was carried out by orally administering extract of at doses of 50, 100 and 200 mg/kg for up to 28 days. The anti-inflammatory and apoptotic properties of the extract in experimental animals were investigated by the expression levels of various genetic biomarkers such as Bax-2, TNF-α, IL-6, COX2, IL-1β, β-Catenin, APC, Bcl2, CEA, Caspase 3 and β-Catenin using reverse transcriptase polymerase chain reaction (RT-PCR).
HPLC analysis shows that has 21 bioactive components which are categorized into seven groups: flavonoid, terpenes, phenols, isoflavonoid, phytosterols, quinone and glycosides. Administration of the drug shows inconsistencies in the mean body weights of the experimental animals. Further investigation revealed that increased TNF-α upregulation and expression, significantly downregulated IL-1β, significantly decreased IL-6 expression, ameliorated COX2 expression, downregulated β-catenin expression and significantly reduced the expression of the APC gene. These results show that the drug activity modulates the investigated inflammatory and apoptotic genes in the prostate gland of PCa-induced rats, thus demonstrating its anti-PCa potential.
The results of this study suggest the potential of a novel treatment protocol of plant extract to improve therapeutic outcomes for patients with aggressive PCa, which reportedly claims hundreds of thousands of lives yearly.
前列腺癌（Pca）是一种在男性中普遍存在的致命疾病，在全球死亡率中占比约7 - 8%。合成药物已被证明有效，但存在局限性和严重副作用。因此，需要发现一种成本更低、无副作用的天然治疗剂来治疗这种疾病。
本研究旨在在实验动物的生理和分子水平上研究（）提取物的抗前列腺癌活性。
对富含多酚的提取物进行高效液相色谱（HPLC）分析，以鉴定该植物的植物化学成分。成年Wistar大鼠口服2毫升50、100和200 ppm的二甲胂酸溶液，持续28天以诱导前列腺癌，同时通过口服给予剂量为50、100和200毫克/千克的提取物进行治疗，持续长达28天。通过使用逆转录聚合酶链反应（RT-PCR）检测各种基因生物标志物如Bax - 2、TNF-α、IL - 6、COX2、IL - 1β、β-连环蛋白、APC、Bcl2、CEA、半胱天冬酶3和β-连环蛋白的表达水平，研究提取物在实验动物中的抗炎和凋亡特性。
HPLC分析表明，（）含有21种生物活性成分，可分为七类：黄酮类、萜类、酚类、异黄酮类、植物甾醇类、醌类和糖苷类。给药后实验动物的平均体重出现不一致情况。进一步研究表明，（）增加了TNF-α的上调和表达，显著下调了IL - 1β，显著降低了IL - 6的表达，改善了COX2的表达，下调了β-连环蛋白的表达，并显著降低了APC基因的表达。这些结果表明，该药物活性调节了前列腺癌诱导大鼠前列腺中所研究的炎症和凋亡基因，从而证明了其抗前列腺癌的潜力。
本研究结果表明，一种新型的（）植物提取物治疗方案有可能改善侵袭性前列腺癌患者的治疗效果，据报道，侵袭性前列腺癌每年夺去数十万人的生命。
