Momina Sayyada Saleha, Gandla Kumaraswamy
Department of Pharmacognosy and Phytochemistry, Chaitanya (Deemed to be University), Gandipet, HimayathNagar (Vill), Hyderabad, Telangana, 500075, India.
Department of Pharmacy, Chaitanya (Deemed to be University), Gandipet, HimayathNagar (Vill), Hyderabad, Telangana, 500075, India.
Biochem Genet. 2025 Apr;63(2):1400-1435. doi: 10.1007/s10528-024-10744-2. Epub 2024 Apr 3.
The present study was aimed at the evaluation of neuroprotective ability of methanolic extract of Trianthema decandra (METD) against hyperglycemia-related cognitive impairment in rats. The extract of T. decandra was standardized by TLC and HPTLC methods. To verify the identity and purity of isolated compounds, they were segregated and characterized using various techniques, including UV-visible spectrophotometry, FT-IR, H-NMR, and Mass spectroscopy. α-Amylase and α-glucosidase inhibition property of the extracts were assessed in-vitro. The screening of the neuroprotective effects of METD in hyperglycemic rats was done utilizing Morri's water (MWM) and elevated plus maze (EPM) model, as well as acetylcholinesterase (AChE) activity. The extracts of Trianthema decandra and its chemical constituents, namely quercetin and phytol, demonstrated a significant protective effect on enzymes like α-amylase and α-glucosidase. Methanol and hydroalcoholic extracts have shown the strongest inhibitory activity followed by chloroform extract. Quercetin and phytol were associated with the methanolic and chloroform extracts which were identified using TLC and HPTLC techniques. During the thirty days of the study, the induction of diabetes in the rats exhibited persistent hyperglycemia, hyperlipidemia, higher escape latency during training trials and reduced time spent in target quadrant in probe trial in Morris water maze test, and increased escape latency in EPM task. Regimen of METD (200 and 400 mg/kg) in the diabetic rats reduced the glucose levels in blood, lipid, and liver profile and showed positive results on Morri's water and elevated plus maze tasks. During the investigation, it was determined that Trianthema decandra extracts and the chemical constituent's quercetin and phytol in it had anti-diabetic and neuroprotective activities.
本研究旨在评估三角车甲醇提取物（METD）对大鼠高血糖相关认知障碍的神经保护能力。三角车提取物通过薄层色谱（TLC）和高效薄层色谱（HPTLC）方法进行标准化。为验证分离化合物的身份和纯度，采用了包括紫外可见分光光度法、傅里叶变换红外光谱法（FT-IR）、氢核磁共振波谱法（H-NMR）和质谱法等多种技术对其进行分离和表征。体外评估了提取物对α-淀粉酶和α-葡萄糖苷酶的抑制特性。利用莫里斯水迷宫（MWM）和高架十字迷宫（EPM）模型以及乙酰胆碱酯酶（AChE）活性，对METD在高血糖大鼠中的神经保护作用进行了筛选。三角车提取物及其化学成分槲皮素和叶绿醇对α-淀粉酶和α-葡萄糖苷酶等酶具有显著的保护作用。甲醇提取物和水醇提取物显示出最强的抑制活性，其次是氯仿提取物。槲皮素和叶绿醇与通过TLC和HPTLC技术鉴定的甲醇提取物和氯仿提取物相关。在为期30天的研究中，大鼠糖尿病诱导表现为持续高血糖、高脂血症、训练试验期间逃避潜伏期延长、莫里斯水迷宫试验中探测试验在目标象限停留时间缩短以及EPM任务中逃避潜伏期增加。糖尿病大鼠给予METD（200和400mg/kg）方案可降低血糖、血脂和肝脏指标水平，并在莫里斯水迷宫和高架十字迷宫任务中显示出阳性结果。在研究过程中，确定三角车提取物及其化学成分槲皮素和叶绿醇具有抗糖尿病和神经保护活性。
