Department of Chemistry, Columbia University, New York, NY, USA.
Kavli Institute for Brain Science, Columbia University, New York, NY, USA.
Nat Methods. 2019 Sep;16(9):830-842. doi: 10.1038/s41592-019-0538-0. Epub 2019 Aug 30.
All molecules consist of chemical bonds, and much can be learned from mapping the spatiotemporal dynamics of these bonds. Since its invention a decade ago, stimulated Raman scattering (SRS) microscopy has become a powerful modality for imaging chemical bonds with high sensitivity, resolution, speed and specificity. We introduce the fundamentals of SRS microscopy and review innovations in SRS microscopes and imaging probes. We highlight examples of exciting biological applications, and share our vision for potential future breakthroughs for this technology.
所有分子都由化学键组成，通过绘制这些化学键的时空动态图，可以了解很多信息。受激拉曼散射（SRS）显微镜自十年前发明以来，已经成为一种具有高灵敏度、高分辨率、高速度和高特异性的化学成像有力手段。我们介绍了 SRS 显微镜的基本原理，并回顾了 SRS 显微镜和成像探针的创新。我们强调了一些令人兴奋的生物学应用实例，并分享了我们对该技术未来潜在突破的展望。
