Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, China.
Jiangnan University Medical Center, Wuxi, China.
Vector Borne Zoonotic Dis. 2024 Jul;24(7):428-438. doi: 10.1089/vbz.2023.0059. Epub 2024 Apr 4.
Clonorchiasis remains a serious public health problem. However, the molecular mechanism underlying clonorchiasis remains largely unknown. Amino acid (AA) metabolism plays key roles in protein synthesis and energy sources, and improves immunity in pathological conditions. Therefore, this study aimed to explore the AA profiles of spleen in clonorchiasis and speculate the interaction between the host and parasite. Here targeted ultrahigh performance liquid chromatography multiple reaction monitoring mass spectrometry was applied to discover the AA profiles in spleen of rats infected with . Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis (KEGG) was performed to characterize the dysregulated metabolic pathways. Pathway analysis revealed that phenylalanine, tyrosine, and tryptophan biosynthesis and β-alanine metabolism were significantly altered in clonorchiasis. There were no significant correlations between 14 significant differential AAs and interleukin (IL)-1β. Although arginine, asparagine, histidine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine were positively correlated with IL-6, IL-10, tumor necrosis factor (TNF)-α as well as aspartate aminotransferase and alanine aminotransferase; β-alanine and 4-hydroxyproline were negatively correlated with IL-6, IL-10, and TNF-α. This study reveals the dysregulation of AA metabolism in clonorchiasis and provides a useful insight of metabolic mechanisms at the molecular level.
华支睾吸虫病仍然是一个严重的公共卫生问题。然而，华支睾吸虫病的分子机制在很大程度上仍然未知。氨基酸（AA）代谢在蛋白质合成和能量来源中起着关键作用，并在病理条件下提高免疫力。因此，本研究旨在探索华支睾吸虫病脾脏中的 AA 谱，并推测宿主与寄生虫之间的相互作用。 本研究采用靶向超高液相色谱多重反应监测质谱法发现感染大鼠脾脏中的 AA 谱。京都基因与基因组百科全书通路富集分析（KEGG）用于表征失调的代谢途径。 通路分析表明，华支睾吸虫病中苯丙氨酸、酪氨酸和色氨酸生物合成和 β-丙氨酸代谢明显改变。14 种差异显著的 AA 与白细胞介素（IL）-1β之间没有显著相关性。虽然精氨酸、天冬酰胺、组氨酸、赖氨酸、蛋氨酸、苯丙氨酸、脯氨酸、丝氨酸、苏氨酸、色氨酸、酪氨酸和缬氨酸与 IL-6、IL-10、肿瘤坏死因子（TNF）-α以及天冬氨酸氨基转移酶和丙氨酸氨基转移酶呈正相关；β-丙氨酸和 4-羟脯氨酸与 IL-6、IL-10 和 TNF-α呈负相关。 本研究揭示了华支睾吸虫病中 AA 代谢的失调，并提供了分子水平上代谢机制的有用见解。
