Han Su, Tang Qiaoran, Lu Xi, Chen Rui, Li Yihong, Shu Jing, Zhang Xiaoli, Cao Jianping
Department of Parasitology, Harbin Medical University, Harbin, 150081, China.
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, Ministry of Health, MOH; National Center for International Research on Tropical Diseases; WHO Collaborating Center for Tropical Diseases, Shanghai, People's Republic of China.
BMC Infect Dis. 2016 Nov 30;16(1):724. doi: 10.1186/s12879-016-2058-1.
Clonorchiasis remains an important zoonotic parasitic disease worldwide. The molecular mechanisms of host-parasite interaction are not fully understood. Non-coding microRNAs (miRNAs) are considered to be key regulators in parasitic diseases. The regulation of miRNAs and host micro-environment may be involved in clonorchiasis, and require further investigation.
MiRNA microarray technology and bioinformatic analysis were used to investigate the regulatory mechanisms of host miRNA and to compare miRNA expression profiles in the liver tissues of control and Clonorchis sinensis (C. sinensis)-infected rats.
A total of eight miRNAs were downregulated and two were upregulated, which showed differentially altered expression profiles in the liver tissue of C. sinensis-infected rats. Further analysis of the differentially expressed miRNAs revealed that many important signal pathways were triggered after infection with C. sinensis, which were related to clonorchiasis pathogenesis, such as cell apoptosis and inflammation, as well as genes involved in signal transduction mechanisms, such as pathways in cancer and the Wnt and Mitogen-activated protein kinases (MAPK) signaling pathways.
The present study revealed that the miRNA expression profiles of the host were changed by C. sinensis infection. This dysregulation in miRNA expression may contribute to the etiology and pathophysiology of clonorchiasis. These results also provide new insights into the regulatory mechanisms of miRNAs in clonorchiasis, which may present potential targets for future C. sinensis control strategies.
华支睾吸虫病仍是全球重要的人畜共患寄生虫病。宿主与寄生虫相互作用的分子机制尚未完全阐明。非编码微小RNA（miRNA）被认为是寄生虫病的关键调节因子。miRNA与宿主微环境的调节可能参与华支睾吸虫病，有待进一步研究。
采用miRNA微阵列技术和生物信息学分析来研究宿主miRNA的调控机制，并比较对照组和感染华支睾吸虫（华支睾吸虫）大鼠肝脏组织中的miRNA表达谱。
共有8种miRNA表达下调，2种miRNA表达上调，在感染华支睾吸虫的大鼠肝脏组织中显示出差异改变的表达谱。对差异表达miRNA的进一步分析表明，感染华支睾吸虫后触发了许多重要的信号通路，这些通路与华支睾吸虫病的发病机制有关，如细胞凋亡和炎症，以及参与信号转导机制的基因，如癌症通路、Wnt和丝裂原活化蛋白激酶（MAPK）信号通路。
本研究表明，华支睾吸虫感染改变了宿主的miRNA表达谱。miRNA表达的这种失调可能有助于华支睾吸虫病的病因学和病理生理学。这些结果也为华支睾吸虫病中miRNA的调控机制提供了新的见解，这可能为未来华支睾吸虫的控制策略提供潜在靶点。
