Zhou Kang-Di, Wang Yu-Jia, Ma Pei-Yan, Fang Shao-Yang, Ma Wei
Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
Int J Biol Macromol. 2025 May;307(Pt 4):142189. doi: 10.1016/j.ijbiomac.2025.142189. Epub 2025 Mar 18.
During meiosis, diploid germ cells undergo two successive rounds of chromosome segregation requiring key changes that sister chromatids co-orient in meiosis I and bi-orient in meiosis II. The kinetochore protein MEIKIN/Moa1 is restricted to meiosis I, has the function to properly co-orient sister kinetochores and maintain pericentrometic cohesion. However, the mechanisms governing the Moa1 activity throughout meiosis remain elusive in Schizosaccharomyces pombe. Here, we demonstrate that fission yeast Moa1 is degraded by the APC/C at anaphase I and blocking Moa1 degradation has no effect on cohesin protection and chromosome segregation during meiosis. Blocking Moa1 degradation can be prevented by the elimination of kinetochore Plo1. Conversely, the removal of Plo1 from the kinetochore, which leads to chromosome mis-segregation, can be reversed by maintaining kinetochore Moa1 levels. Therefore, we have observed a feedback relationship between reduced Plo1 enrichment at kinetochores and inhibited Moa1 degradation.
在减数分裂过程中，二倍体生殖细胞经历两轮连续的染色体分离，这需要关键的变化，即姐妹染色单体在减数第一次分裂中共同定向，在减数第二次分裂中双向定向。动粒蛋白MEIKIN/Moa1仅限于减数第一次分裂，具有使姐妹动粒正确共同定向并维持着丝粒周围黏连的功能。然而，在粟酒裂殖酵母中，整个减数分裂过程中调控Moa1活性的机制仍不清楚。在这里，我们证明裂殖酵母Moa1在减数第一次分裂后期被后期促进复合物/细胞周期体（APC/C）降解，并且在减数分裂期间阻断Moa1降解对黏连蛋白保护和染色体分离没有影响。消除动粒Plo1可以防止阻断Moa1降解。相反，通过维持动粒Moa1水平，可以逆转因从动粒上去除Plo1而导致的染色体错误分离。因此，我们观察到动粒处Plo1富集减少与Moa1降解受抑制之间存在反馈关系。
