State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, China.
College of Life Sciences, Wuhan University, Wuhan 430072, China.
Int J Mol Sci. 2022 Jun 24;23(13):7045. doi: 10.3390/ijms23137045.
As a sessile organism, rice often faces various kinds of abiotic stresses, such as drought stress. Drought stress seriously harms plant growth and damages crop yield every year. Therefore, it is urgent to elucidate the mechanisms of drought resistance in rice. In this study, we identified a glycine-rich RNA-binding protein, OsGRP3, in rice. Evolutionary analysis showed that it was closely related to OsGR-RBP4, which was involved in various abiotic stresses. The expression of OsGRP3 was shown to be induced by several abiotic stress treatments and phytohormone treatments. Then, the drought tolerance tests of transgenic plants confirmed that OsGRP3 enhanced drought resistance in rice. Meanwhile, the yeast two-hybrid assay, bimolecular luminescence complementation assay and bimolecular fluorescence complementation assay demonstrated that OsGRP3 bound with itself may affect the RNA chaperone function. Subsequently, the RNA-seq analysis, physiological experiments and histochemical staining showed that OsGRP3 influenced the phenylpropanoid biosynthesis pathway and further modulated lignin accumulation. Herein, our findings suggested that OsGRP3 enhanced drought resistance in rice by altering the phenylpropanoid biosynthesis pathway and further increasing lignin accumulation.
作为一种固着生物，水稻经常面临各种非生物胁迫，如干旱胁迫。干旱胁迫严重危害植物生长，每年损害作物产量。因此，阐明水稻的抗旱机制迫在眉睫。在这项研究中，我们在水稻中鉴定了一种甘氨酸丰富的 RNA 结合蛋白 OsGRP3。进化分析表明，它与参与各种非生物胁迫的 OsGR-RBP4 密切相关。结果表明，OsGRP3 的表达受几种非生物胁迫处理和植物激素处理的诱导。然后，对转基因植物的耐旱性测试证实，OsGRP3 增强了水稻的耐旱性。同时，酵母双杂交分析、双分子荧光互补分析和双分子荧光互补分析表明，OsGRP3 可能通过自身结合来影响 RNA 伴侣功能。随后，RNA-seq 分析、生理实验和组织化学染色表明，OsGRP3 影响了苯丙烷生物合成途径，并进一步调节木质素积累。因此，我们的研究结果表明，OsGRP3 通过改变苯丙烷生物合成途径并进一步增加木质素积累来增强水稻的耐旱性。
