College of Life Sciences, Jilin Agricultural University, Changchun 130118, China.
College of Agronomy, Jilin Agricultural University, Changchun 130118, China.
Int J Mol Sci. 2022 Dec 15;23(24):15984. doi: 10.3390/ijms232415984.
The papain-like cysteine proteases (PLCPs) is a subfamily of cysteine proteases that plays an important role in leaf senescence, and some of its members are involved in the regulation of plant growth and development under stress. In this study, we cloned a new gene, , from maize. Expression profile analysis showed that was induced by darkness and drought treatments. In addition, the overexpression in maize accelerated the senescence of maize leaves under darkness and drought treatments. However, the knockout of in maize enhanced the resistance of maize to darkness and drought stresses and reduced the degree of senescence of maize leaves. Under drought stress, compared with WT plants, the knockout lines had a higher seed germination rate, seedling survival rate and chlorophyll content, and lower reactive oxygen species (ROS) level and malondialdehyde (MDA) content. In addition, quantitative real-time PCR (qRT-PCR) analysis showed that negatively regulated some stress-related genes but positively regulated senescence-related genes under darkness and drought stress conditions. To summarize, these results indicate that is a senescence-related gene and plays a negative role in response to darkness and drought stresses. This study laid a theoretical foundation for the innovation of maize germplasm resources with high quality, high yield and strong stress resistance.
木瓜蛋白酶样半胱氨酸蛋白酶（PLCPs）是半胱氨酸蛋白酶家族的一个亚家族，在叶片衰老过程中发挥重要作用，其部分成员参与逆境下植物生长发育的调控。本研究从玉米中克隆了一个新基因 。表达谱分析表明， 受黑暗和干旱处理诱导。此外，玉米中 的过表达加速了黑暗和干旱处理下玉米叶片的衰老。然而，玉米中 的敲除增强了玉米对黑暗和干旱胁迫的抗性，降低了玉米叶片衰老的程度。在干旱胁迫下，与 WT 植株相比，敲除系具有更高的种子发芽率、幼苗成活率和叶绿素含量，以及更低的活性氧（ROS）水平和丙二醛（MDA）含量。此外，定量实时 PCR（qRT-PCR）分析表明， 在黑暗和干旱胁迫条件下， 负调控一些与胁迫相关的基因，但正调控与衰老相关的基因。综上所述，这些结果表明 是一个与衰老相关的基因，在响应黑暗和干旱胁迫中起负调控作用。本研究为培育具有优质、高产和强抗逆性的玉米种质资源提供了理论基础。
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