姜黄素通过 CLDN7、CLDN11 和 CD274 m6A 甲基化修饰抑制卵巢癌细胞 SKOV3 的侵袭。

Ginger inhibits the invasion of ovarian cancer cells SKOV3 through CLDN7, CLDN11 and CD274 m6A methylation modifications.

机构信息

School of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China.

Department of Obstetrics and Gynecology, Shandong Provincial Third Hospital, Jinan, 250031, P.R. China.

出版信息

BMC Complement Med Ther. 2024 Apr 4;24(1):145. doi: 10.1186/s12906-024-04431-3.

DOI:10.1186/s12906-024-04431-3

PMID:38575994

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10993429/

Abstract

BACKGROUND

Ginger is a common aromatic vegetable with a wide range of functional ingredients and considerable medicinal and nutritional properties. Numerous studies have shown that ginger and its active ingredients have suppressive effects on manifold tumours, including ovarian cancer (OC). However, the molecular mechanism by which ginger inhibits OC is not clear. The aim of this study was to investigate the function and mechanism of ginger in OC.

METHODS

The estimation of n6-methyladenosine (m6A) levels was performed using the m6A RNA Methylation Quantification Kit, and RT-qPCR was used to determine the expression of m6A-related genes and proteins. The m6A methylationome was detected by MeRIP-seq, following analysis of the data. Differential methylation of genes was assessed utilizing RT-qPCR and Western Blotting. The effect of ginger on SKOV3 invasion in ovarian cancer cells was investigated using the wound healing assay and transwell assays.

RESULTS

Ginger significantly reduced the m6A level of OC cells SKOV3. The 3'UTR region is the major site of modification for m6A methylation, and its key molecular activities include Cell Adhesion Molecules, according to meRIP-seq results. Moreover, it was observed that Ginger aids significantly in downregulating the CLDN7, CLDN11 mRNA, and protein expression. The results of wound healing assay and transwell assay showed that ginger significantly inhibited the invasion of OC cells SKOV3.

CONCLUSIONS

Ginger inhibits ovarian cancer cells' SKOV3 invasion by regulating m6A methylation through CLDN7, CLDN11, and CD274.

摘要

背景

生姜是一种常见的芳香蔬菜，含有多种功能成分，具有相当的药用和营养价值。大量研究表明，生姜及其活性成分对多种肿瘤具有抑制作用，包括卵巢癌（OC）。然而，生姜抑制 OC 的分子机制尚不清楚。本研究旨在探讨生姜在 OC 中的作用和机制。

方法

采用 m6A RNA 甲基化定量试剂盒评估 n6-甲基腺苷（m6A）水平，采用 RT-qPCR 测定 m6A 相关基因和蛋白的表达。通过 MeRIP-seq 检测 m6A 甲基化组，对数据进行分析。利用 RT-qPCR 和 Western Blotting 评估基因的差异甲基化。采用划痕愈合实验和 Transwell 实验研究生姜对卵巢癌细胞 SKOV3 侵袭的影响。

结果

生姜显著降低了 OC 细胞 SKOV3 的 m6A 水平。MeRIP-seq 结果表明，m6A 甲基化的主要修饰部位在 3'UTR 区域，其关键分子活性包括细胞黏附分子。此外，研究还发现生姜有助于显著下调 CLDN7、CLDN11 mRNA 和蛋白的表达。划痕愈合实验和 Transwell 实验的结果表明，生姜显著抑制了 OC 细胞 SKOV3 的侵袭。