Widjiastuti Ira, Kunarti Sri, Ningtyas Evri Kusumah, Atikah Ayu Rafania, Basalamah Khadijah Fauzi, Puspita Amelia Evita, Widianti Novelia Ayu, Noor Tengku Natasha Eleena Binti Tengku Ahmad
Department of Conservative Dentistry, Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia.
Malaysian Armed Forces Dental Officer, 609 Armed Forces Dental Clinic, Kem Semenggo, Kuching, Sarawak, Malaysia.
Iran Endod J. 2024;19(2):99-104. doi: 10.22037/iej.v19i2.42269.
This study explored the effects of propolis extract calcium hydroxide (Ca(OH)) combination on malondialdehyde and superoxide dismutase expression in dental pulp, aiming to assess its potential as a direct pulp capping material.
Thirty male Wistar rats were randomly assigned to three groups. Mandibular molar teeth were prepared using a low-speed round bur. In Group I, no material was applied; in Group II, teeth were treated with Ca(OH); and in Group III, teeth were treated with Propolis extract-Ca(OH), followed by Cention N filling. Immunohistochemistry was conducted on pulp tissue samples obtained on the third and seventh days post-treatment to assess malondialdehyde and superoxide dismutase expression. Statistical analyses included the Shapiro-Wilk test, Levene test, ANOVA, and Tukey's HSD.
The samples treated with propolis extract-Ca(OH) combination exhibited significantly lower malondialdehyde expression on both days compared to samples treated with Ca(OH) (<0.05), indicating reduced oxidative stress. Superoxide dismutase expression in the propolis extract-Ca(OH) group was higher (<0.05), suggesting an enhanced antioxidant activity. The control group showed intermediate results. Statistical analyses confirmed significant differences between groups for both malondialdehyde and superoxide dismutase expressions (<0.05).
The study suggests that the propolis extract-Ca(OH) combination holds promise for direct pulp capping applications by minimizing oxidative stress and promoting antioxidant defense mechanisms in dental pulp.
本研究探讨了蜂胶提取物与氢氧化钙（Ca(OH)₂）联合应用对牙髓中丙二醛和超氧化物歧化酶表达的影响，旨在评估其作为直接盖髓材料的潜力。
将30只雄性Wistar大鼠随机分为三组。使用低速圆钻制备下颌磨牙。第一组不应用任何材料；第二组用Ca(OH)₂处理牙齿；第三组用蜂胶提取物 - Ca(OH)₂处理牙齿，然后用Cention N进行充填。在治疗后第3天和第7天获取牙髓组织样本进行免疫组织化学分析，以评估丙二醛和超氧化物歧化酶的表达。统计分析包括Shapiro-Wilk检验、Levene检验、方差分析和Tukey's HSD检验。
与用Ca(OH)₂处理的样本相比，用蜂胶提取物 - Ca(OH)₂联合处理的样本在这两天的丙二醛表达均显著降低（<0.05），表明氧化应激减轻。蜂胶提取物 - Ca(OH)₂组的超氧化物歧化酶表达较高（<0.05），表明抗氧化活性增强。对照组显示出中间结果。统计分析证实，丙二醛和超氧化物歧化酶表达在各组之间存在显著差异（<0.05）。
该研究表明，蜂胶提取物 - Ca(OH)₂联合应用有望通过最小化氧化应激和促进牙髓中的抗氧化防御机制用于直接盖髓应用。
