伊朗蜂胶与氢氧化钙对牙髓成纤维细胞的细胞毒性评估
Cytotoxicity evaluation of Iranian propolis and calcium hydroxide on dental pulp fibroblasts.
作者信息
Zare Jahromi Maryam, Ranjbarian Parisa, Shiravi Samaneh
机构信息
Assistant Professor, Department of Endodontics, Faculty of Dentistry, Islamic Azad University Khorasgan (Isfahan) Branch, Isfahan, Iran.
Post-graduate Student, Department of Endodontics, Faculty of Dentistry, Islamic Azad University, Khorasgan (Isfahan) Branch, Isfahan, Iran.
出版信息
J Dent Res Dent Clin Dent Prospects. 2014 Summer;8(3):130-3. doi: 10.5681/joddd.2014.024. Epub 2014 Sep 17.
Abstract
Background and aims. Since intracanal medicaments can affect the cell viability in periapical tissues, the aim of this study was to evaluate the effect of calcium hydroxide and propolis on pulp fibroblasts. Materials and methods. Two healthy third molars were used as a source to obtain fibroblasts. The fibroblasts were cultured and subjected to 1 mg/mL of propolis and calcium hydroxide. This experiment was performed in six replicates and cell viability was evaluated with MTT assay. Statistical analysis was performed by t-test. Results. Comparison of cell viability with the use of 1 mg/mL of calcium hydroxide and propolis showed that cells subjected to propolis were more viable when compared to calcium hydroxide (P < 0.05). Conclusion. In this study, calcium hydroxide reduced fibroblast viability, significantly more than Iranian propolis. Other properties should be evaluated before Iranian propolis could be indicated for use as intracanal medicament.
摘要
背景与目的。由于根管内药物可影响根尖周组织中的细胞活力，本研究旨在评估氢氧化钙和蜂胶对牙髓成纤维细胞的影响。材料与方法。使用两颗健康的第三磨牙作为获取成纤维细胞的来源。对成纤维细胞进行培养，并使其分别接触1mg/mL的蜂胶和氢氧化钙。本实验重复进行六次，并采用MTT法评估细胞活力。通过t检验进行统计分析。结果。使用1mg/mL氢氧化钙和蜂胶时细胞活力的比较显示，与氢氧化钙相比，接触蜂胶的细胞活力更强（P < 0.05）。结论。在本研究中，氢氧化钙降低了成纤维细胞的活力，且显著超过伊朗蜂胶。在伊朗蜂胶可被用作根管内药物之前，还应评估其其他特性。
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