Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Airlangga University, Surabaya, East Java, 60131, Indonesia.
Department of Conservative Dentistry, Faculty of Dentistry, Airlangga University, Surabaya, East Java, 60131, Indonesia.
F1000Res. 2020 Apr 29;9:308. doi: 10.12688/f1000research.22409.1. eCollection 2020.
Caries in the dental pulp result in inflammation and damage to the pulp tissue. During inflammation of the pulp, various inflammatory mediators and growth factors are released, including IL-8, IL-10, TLR-2, VEGF and TGF-β through the NF-kB pathway. In the present study, therapy for pulpal caries was performed through pulp capping by giving a combination of propolis and calcium hydroxide (Ca(OH)2). This treatment was expected to stimulate the formation of reparative dentin as an anti-inflammatory material to prevent pulp tissue damage. 28 Wistar rats were divided into four groups and treated with Ca(OH)2 with or without the addition of propolis for either 7 or 14 days. Immunohistochemical examination was used to determine the expression of IL-8, IL-10, TLR-2, VEGF, TGF-β in the four treatment groups. The group treated with a combination of propolis and Ca(OH)2 for 7 days showed that the expression of IL-10, IL-8, TLR-2, VEGF, TGF-β increased significantly compared to the treatment group treated with only Ca(OH)2. The expression of IL-10, TLR-2, TGF-β, VEGF increased in the treatment group treated with propolis and Ca(OH)2 for 14 days, while the expression of IL-8 in the decreased significantly. Administration of a combination of propolis and Ca(OH)2 has efficacy in the pulp capping treatment process because it has anti-bacterial and immunomodulatory properties. The results show that it is able to stimulate the process of pulp tissue repair through increased expression of IL-10, TGF-β, VEGF, TLR -2 and decreased expression of IL-8.
牙髓龋导致牙髓组织炎症和损伤。在牙髓炎症期间，通过 NF-kB 途径释放各种炎症介质和生长因子，包括 IL-8、IL-10、TLR-2、VEGF 和 TGF-β。在本研究中，通过给予蜂胶和氢氧化钙 (Ca(OH)2) 的组合进行牙髓龋治疗来进行牙髓治疗。这种治疗预计会刺激形成修复性牙本质作为抗炎物质，以防止牙髓组织损伤。 28 只 Wistar 大鼠分为四组，分别用 Ca(OH)2 加或不加蜂胶处理 7 或 14 天。采用免疫组织化学法检测四组治疗中 IL-8、IL-10、TLR-2、VEGF、TGF-β 的表达。 用蜂胶和 Ca(OH)2 联合处理 7 天的组与仅用 Ca(OH)2 处理的组相比，IL-10、IL-8、TLR-2、VEGF、TGF-β 的表达明显增加。用蜂胶和 Ca(OH)2 处理 14 天的治疗组中 IL-10、TLR-2、TGF-β、VEGF 的表达增加，而 IL-8 的表达明显减少。 蜂胶和 Ca(OH)2 的联合给药在牙髓盖髓治疗过程中有效，因为它具有抗菌和免疫调节特性。结果表明，它能够通过增加 IL-10、TGF-β、VEGF、TLR-2 的表达和减少 IL-8 的表达来刺激牙髓组织修复过程。
