用于检测细胞表面抗原标志物的红细胞连接抗体检测法。
Red cell-linked antibody assay for detection of antigenic markers on cell surfaces.
作者信息
Mihaescu S, Sjöquist J, Gheţie V
出版信息
J Immunol Methods. 1979;29(1):79-83. doi: 10.1016/0022-1759(79)90128-5.
PMID:385784
Abstract
A direct method for demonstrating antigenic markers on cell surface has been developed which makes use of the ability of bovine red blood cells coated with protein A of Staphylococcus aureus (ES) to binding IgG antibody without agglutinating. Red cell-linked rabbit IgG antibody reagents (ESA) were prepared in this way, and Ig-, IgM- and Thy-1-bearing mouse spleen and peripheral blood lymphocytes were identified by a rosette assay.
摘要
已开发出一种直接显示细胞表面抗原标记的方法，该方法利用了包被有金黄色葡萄球菌A蛋白（ES）的牛红细胞结合IgG抗体而不发生凝集的能力。通过这种方式制备了红细胞连接的兔IgG抗体试剂（ESA），并通过玫瑰花环试验鉴定了带有Ig、IgM和Thy-1的小鼠脾细胞和外周血淋巴细胞。
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