Romagnani S, Del Prete G F, Giudizi G M, Almerigogna F, Ricci M
J Immunol Methods. 1979;29(3):263-70. doi: 10.1016/0022-1759(79)90314-4.
A radioimmunoassay is described for measuring IgG, based on the ability of immunoglobulins of this class to inhibit the binding of radioiodinated staphylococcal protein A to IgG linked to a solid phase. The solid phase is represented by ox erythrocytes coated with anti-ox erythrocyte rabbit IgG, a reagent used for detecting cells equipped with receptors for the Fc fragment of IgG. By this assay the IgG secreted in vitro by human peripheral blood lymphocytes stimulated with PWM and those present in samples of very diluted human sera were measured. It was found that the assay is a very rapid, simple and reproducible procedure for the detection of IgG immunoglobulin at the nanogram level.
本文描述了一种用于测量IgG的放射免疫测定法，该方法基于此类免疫球蛋白抑制放射性碘化葡萄球菌蛋白A与连接到固相的IgG结合的能力。固相由包被有抗牛红细胞兔IgG的牛红细胞代表，抗牛红细胞兔IgG是一种用于检测配备有IgG Fc片段受体的细胞的试剂。通过该测定法，测量了用PWM刺激的人外周血淋巴细胞体外分泌的IgG以及非常稀释的人血清样品中存在的IgG。结果发现，该测定法是一种用于检测纳克水平IgG免疫球蛋白的非常快速、简单且可重复的方法。
