Moţa G, Sjöquist J, Gheţie V
Mol Immunol. 1984 Jul;21(7):641-5. doi: 10.1016/0161-5890(84)90049-x.
By reacting rabbit IgG with the fragment AB of protein A from S. aureus (mol. wt 14,000) an IgG dimer was formed with an approx. mol. wt of 320,000 and a molar composition of IgG2-AB1. A hybrid dimer with dual specificity consisting of IgG anti-sheep red blood cells/AB/IgG anti-bovine red blood cells was also obtained by reacting successively both rabbit antibodies with the AB fragment. The immunologic properties (affinity for antigen, complement activation and binding to Fc receptors) of the dimeric IgG were investigated in comparison with monomeric rabbit IgG and a tetrameric IgG obtained by reaction with protein A, (IgG2-protein A1)2.
通过使兔免疫球蛋白G（IgG）与来自金黄色葡萄球菌的A蛋白片段AB（分子量14,000）反应，形成了一种IgG二聚体，其分子量约为320,000，摩尔组成为IgG2-AB1。通过使两种兔抗体依次与AB片段反应，还获得了一种具有双重特异性的杂合二聚体，其由抗绵羊红细胞IgG/AB/抗牛红细胞IgG组成。与单体兔IgG以及通过与A蛋白反应获得的四聚体IgG（IgG2-A蛋白1）2相比，对二聚体IgG的免疫学特性（对抗原的亲和力、补体激活以及与Fc受体的结合）进行了研究。
