Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Sci Data. 2024 Apr 5;11(1):340. doi: 10.1038/s41597-024-03189-0.
Despite the rapid advances in sequencing technology, limited genomic resources are currently available for phytophagous spider mites, which include many important agricultural pests. One of these pests is Tetranychus piercei (McGregor), a serious banana pest in East Asia exhibiting remarkable tolerance to high temperature. In this study, we assembled a high-quality genome of T. piercei using a combination of PacBio long reads and Illumina short reads sequencing. With the assistance of chromatin conformation capture technology, 99.9% of the contigs were anchored into three pseudochromosomes with a total size of 86.02 Mb. Repetitive elements, accounting for 14.16% of this genome (12.20 Mb), are predominantly composed of long-terminal repeats (30.7%). By combining evidence of ab initio prediction, transcripts, and homologous proteins, we annotated 11,881 protein-coding genes. Both the genome and proteins have high BUSCO completeness scores (>94%). This high-quality genome, along with reliable annotation, provides a valuable resource for investigating the high-temperature tolerance of this species and exploring the genomic basis that underlies the host range evolution of spider mites.
尽管测序技术发展迅速，但目前用于植食性蜘蛛螨的基因组资源有限，而蜘蛛螨包括许多重要的农业害虫。其中一种害虫是二斑叶螨（Tetranychus piercei）（McGregor），它是东亚地区一种严重的香蕉害虫，对高温具有显著的耐受性。在这项研究中，我们结合 PacBio 长读长和 Illumina 短读长测序技术，组装了高质量的二斑叶螨基因组。在染色质构象捕获技术的辅助下，99.9%的序列被锚定到三个假染色体上，总大小为 86.02 Mb。重复元件占该基因组的 14.16%（12.20 Mb），主要由长末端重复（30.7%）组成。通过结合从头预测、转录本和同源蛋白的证据，我们注释了 11881 个编码蛋白的基因。基因组和蛋白质都具有很高的 BUSCO 完整性分数（>94%）。这个高质量的基因组，以及可靠的注释，为研究该物种对高温的耐受性以及探索蜘蛛螨宿主范围进化的基因组基础提供了有价值的资源。
