二斑叶螨基因组的染色体水平组装。
Chromosome-level genome assembly of the two-spotted spider mite Tetranychus urticae.
机构信息
College of Plant Protection, South China Agricultural University, Guangzhou, China.
Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
出版信息
Sci Data. 2024 Jul 18;11(1):798. doi: 10.1038/s41597-024-03640-2.
The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is a notorious pest in agriculture that has developed resistance to almost all chemical types used for its control. Here, we assembled a chromosome-level genome for the TSSM using Illumina, Nanopore, and Hi-C sequencing technologies. The assembled contigs had a total length of 103.94 Mb with an N50 of 3.46 Mb, with 87.7 Mb of 34 contigs anchored to three chromosomes. The chromosome-level genome assembly had a BUSCO completeness of 94.8%. We identified 15,604 protein-coding genes, with 11,435 genes that could be functionally annotated. The high-quality genome provides invaluable resources for the genetic and evolutionary study of TSSM.
二斑叶螨，Tetranychus urticae Koch（蛛形纲：叶螨科），是农业中一种声名狼藉的害虫，几乎对所有用于防治它的化学类型都产生了抗性。在这里，我们使用 Illumina、Nanopore 和 Hi-C 测序技术为 TSSM 组装了一个染色体水平的基因组。组装的 contigs 总长度为 103.94 Mb，N50 为 3.46 Mb，其中 34 个 contigs 的 87.7 Mb 锚定到三个染色体上。染色体水平的基因组组装的 BUSCO 完整性为 94.8%。我们鉴定了 15,604 个蛋白质编码基因，其中 11,435 个基因可以进行功能注释。高质量的基因组为 TSSM 的遗传和进化研究提供了宝贵的资源。
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