College of Plant Protection, South China Agricultural University, Guangzhou, China.
Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Sci Data. 2024 Jul 18;11(1):798. doi: 10.1038/s41597-024-03640-2.
The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is a notorious pest in agriculture that has developed resistance to almost all chemical types used for its control. Here, we assembled a chromosome-level genome for the TSSM using Illumina, Nanopore, and Hi-C sequencing technologies. The assembled contigs had a total length of 103.94 Mb with an N50 of 3.46 Mb, with 87.7 Mb of 34 contigs anchored to three chromosomes. The chromosome-level genome assembly had a BUSCO completeness of 94.8%. We identified 15,604 protein-coding genes, with 11,435 genes that could be functionally annotated. The high-quality genome provides invaluable resources for the genetic and evolutionary study of TSSM.
二斑叶螨，Tetranychus urticae Koch（蛛形纲：叶螨科），是农业中一种声名狼藉的害虫，几乎对所有用于防治它的化学类型都产生了抗性。在这里，我们使用 Illumina、Nanopore 和 Hi-C 测序技术为 TSSM 组装了一个染色体水平的基因组。组装的 contigs 总长度为 103.94 Mb，N50 为 3.46 Mb，其中 34 个 contigs 的 87.7 Mb 锚定到三个染色体上。染色体水平的基因组组装的 BUSCO 完整性为 94.8%。我们鉴定了 15,604 个蛋白质编码基因，其中 11,435 个基因可以进行功能注释。高质量的基因组为 TSSM 的遗传和进化研究提供了宝贵的资源。
