Hacettepe University, Faculty of Science, Department of Chemistry, Ankara, Turkey.
Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, Ankara, Turkey.
Bioelectrochemistry. 2024 Aug;158:108701. doi: 10.1016/j.bioelechem.2024.108701. Epub 2024 Apr 3.
Herein, we proposed a new approach to design a MIP-based electrochemical sensor with carbon nanofiber (CNF), which could improve its conductivities as well as electrode sensitivity and successful detection of dasatinib (DAS). CNFs are capable of forming high porosity with significant interconnected porous networks. The poly(2-hydroxyethyl-methacrylate-N-methacryloyl-L-tyrosine) (PHEMA-MATyr) copolymer was synthesized in the presence of both CNF and DAS by photopolymerization. After optimization of the parameters, the modified MIP-based electrochemical sensor demonstrated the ability to determine the DAS in the linear working range of 1.0 × 10-1.0 × 10 M for the standard solution and commercial serum samples with a LOD of 1.76 × 10 and 2.46 × 10, respectively. Good linearity for DAS was observed with correlation coefficients (r) of 0.996 and 0.997 for the standard solution and commercial serum samples, respectively. The recoveries of the DAS ranged from 99.45 % to 99.53 % for the tablet dosage form and commercial serum samples, with average relative standard deviations below 1.96 % in both cases. The proposed modified sensor demonstrated significant sensitivity and selectivity for the rapid determination of DAS in commercial serum samples and tablet form.
在此，我们提出了一种新的方法来设计基于介孔分子筛的电化学传感器，该传感器采用碳纤维（CNF），可以提高其电导率以及电极灵敏度，成功检测达沙替尼（DAS）。CNF 能够形成具有显著相互连通的多孔网络的高多孔性。在 CNF 和 DAS 的存在下，通过光聚合合成了聚（2-羟乙基甲基丙烯酸甲酯- N-丙烯酰基-L-酪氨酸）（PHEMA-MATyr）共聚物。在优化参数后，修饰后的基于 MIP 的电化学传感器表现出在 1.0×10-1.0×10 M 的标准溶液和商业血清样品的线性工作范围内测定 DAS 的能力，检测限分别为 1.76×10和 2.46×10。对于标准溶液和商业血清样品，DAS 的线性相关性良好，相关系数（r）分别为 0.996 和 0.997。对于片剂剂型和商业血清样品，DAS 的回收率范围为 99.45%-99.53%，两种情况下的平均相对标准偏差均低于 1.96%。所提出的改良传感器在商业血清样品和片剂形式中对 DAS 的快速测定表现出显著的灵敏度和选择性。
