State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu 730020, China.
Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China.
J Genet Genomics. 2024 Aug;51(8):866-876. doi: 10.1016/j.jgg.2024.03.015. Epub 2024 Apr 4.
The phenotypic diversity resulting from artificial or natural selection of sheep has made a significant contribution to human civilization. Hu sheep are a local sheep breed unique to China with high reproductive rates and rapid growth. Genomic selection signatures have been widely used to investigate the genetic mechanisms underlying phenotypic variation in livestock. Here, we conduct whole-genome sequencing of 207 Hu sheep and compare them with the wild ancestors of domestic sheep (Asiatic mouflon) to investigate the genetic characteristics and selection signatures of Hu sheep. Based on six signatures of selection approaches, we detect genomic regions containing genes related to reproduction (BMPR1B, BMP2, PGFS, CYP19, CAMK4, GGT5, and GNAQ), vision (ALDH1A2, SAG, and PDE6B), nervous system (NAV1), and immune response (GPR35, SH2B2, PIK3R3, and HRAS). Association analysis with a population of 1299 Hu sheep reveals that those missense mutations in the GPR35 (GPR35 g.952651 A>G; GPR35 g.952496 C>T) and NAV1 (NAV1 g.84216190 C>T; NAV1 g.84227412 G>A) genes are significantly associated (P < 0.05) with immune and growth traits in Hu sheep, respectively. This research offers unique insights into the selection characteristics of Hu sheep and facilitates further genetic improvement and molecular investigations.
绵羊的人工或自然选择所产生的表型多样性对人类文明做出了重大贡献。湖羊是中国特有的一种地方绵羊品种，具有繁殖率高、生长速度快的特点。基因组选择特征已被广泛用于研究家畜表型变异的遗传机制。在这里，我们对 207 只湖羊进行了全基因组测序，并将其与绵羊的野生祖先（亚洲盘羊）进行了比较，以研究湖羊的遗传特征和选择特征。基于 6 种选择方法的特征，我们检测到了包含与繁殖（BMPR1B、BMP2、PGFS、CYP19、CAMK4、GGT5 和 GNAQ）、视觉（ALDH1A2、SAG 和 PDE6B）、神经系统（NAV1）和免疫反应（GPR35、SH2B2、PIK3R3 和 HRAS）相关的基因的基因组区域。对 1299 只湖羊群体的关联分析表明，GPR35（GPR35 g.952651 A>G；GPR35 g.952496 C>T）和 NAV1（NAV1 g.84216190 C>T；NAV1 g.84227412 G>A）基因中的这些错义突变与湖羊的免疫和生长性状分别显著相关（P < 0.05）。这项研究为湖羊的选择特征提供了独特的见解，并有助于进一步的遗传改良和分子研究。
