不同的化学成分和酶处理可诱导人内皮细胞在脱细胞羊主动脉中存活。
Distinct chemical composition and enzymatic treatment induced human endothelial cells survival in acellular ovine aortae.
作者信息
Heidarzadeh Morteza, Rahbarghazi Reza, Saberianpour Shirin, Delkhosh Aref, Amini Hassan, Sokullu Emel, Hassanpour Mehdi
机构信息
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Koç University Translational Medicine Research Center (KUTTAM) Rumeli Feneri, Sarıyer, Istanbul, Turkey.
出版信息
BMC Res Notes. 2021 Apr 7;14(1):126. doi: 10.1186/s13104-021-05538-3.
OBJECTIVE
The current experiment aimed to assess the impact of detergents such as 3% Triton X-100, 1% peracetic acid, 1% Tween-20, and 1% SDS in combination with Trypsin-EDTA on acellularization of ovine aortae after 7 days.
RESULTS
Hematoxylin-Eosin staining showed an appropriate acellularization rate in ovine aortae, indicated by a lack of cell nuclei in the tunica media layer. DAPI staining confirmed the lack of nuclei in the vascular wall after being exposed to the combination of chemical and enzymatic solutions. Verhoeff-Van Gieson staining showed that elastin fibers were diminished in acellular samples compared to the control group while collagen stands were unchanged. CCK-8 survival assay showed enhanced viability in human umbilical vein endothelial cells 5 days after being cultured on decellularized samples compared to the cells cultured on a plastic surface (p < 0.05). SEM imaging showed flattening of endothelial cells on the acellular surface.
目的
当前实验旨在评估3% Triton X-100、1%过氧乙酸、1%吐温-20和1%十二烷基硫酸钠等去污剂与胰蛋白酶-乙二胺四乙酸联合使用对羊主动脉脱细胞7天后的影响。
结果
苏木精-伊红染色显示羊主动脉脱细胞率合适，表现为中膜层无细胞核。DAPI染色证实，在暴露于化学和酶溶液的组合后，血管壁中无细胞核。Verhoeff-Van Gieson染色显示，与对照组相比，脱细胞样本中的弹性纤维减少，而胶原纤维束未改变。CCK-8存活试验显示，与在塑料表面培养的细胞相比，在脱细胞样本上培养5天后，人脐静脉内皮细胞的活力增强（p < 0.05）。扫描电子显微镜成像显示脱细胞表面的内皮细胞变平。
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