乳酸菌生物转化杏仁提取物的抗菌、抗氧化活性
Antibacterial, antioxidant activities of lactic acid bacteria-bioconversioned almond extract.
作者信息
Lee Kiha, Lee Daehyeop, Lee Geonhak, Lee Woorin, Lee Kwang-Geun
机构信息
Department of Food Science and Biotechnology, Dongguk University-Seoul, 32, Dongguk-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 400-820 Republic of Korea.
出版信息
Food Sci Biotechnol. 2023 Nov 28;33(6):1487-1493. doi: 10.1007/s10068-023-01450-7. eCollection 2024 May.
This study was about bioconversion of almonds by lactic acid bacteria. There are two bacteria used for bioconversion of almond extract: ATCC14917 and GG KCTC5033. Almond extract (AE) was inoculated with or GG for 3 days. AE inoculated with (LP-AE) and AE inoculated with GG (LR-AE) inhibited the growth of Typhimurium and the growth of by 30 and 75%, respectively compared to AE. LR-AE inhibited 30% of biofilm formation of Typhimurium and compared to AE. Antioxidative activity of LP-AE and LR-AE was determined using the 2,2'-zino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) assay. ABTS radical scavenging activity of AE, LP-AE and LR-AE was 53.50%, 80.39% and 83.57%, respectively. The highest level of total polyphenol (89.72 mg Gallic Acid Equivalent (GAE)/g) and flavonoid (194.56 mg Quercetin Equivalent (QCE)/g) contents was found in LR-AE.
本研究是关于乳酸菌对杏仁的生物转化。有两种细菌用于杏仁提取物的生物转化：ATCC14917和GG KCTC5033。杏仁提取物（AE）分别接种或GG，培养3天。与AE相比，接种的AE（LP - AE）和接种GG的AE（LR - AE）分别抑制了鼠伤寒沙门氏菌和的生长30%和75%。与AE相比，LR - AE抑制了鼠伤寒沙门氏菌30%的生物膜形成。使用2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）法测定LP - AE和LR - AE的抗氧化活性。AE、LP - AE和LR - AE的ABTS自由基清除活性分别为53.50%、80.39%和83.57%。在LR - AE中发现总多酚（89.72毫克没食子酸当量（GAE）/克）和类黄酮（194.56毫克槲皮素当量（QCE）/克）含量最高。
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