NCFM 和 Lp-115 抑制了小鼠模型中的定植和胃炎症。

NCFM and Lp-115 inhibit colonization and gastric inflammation in a murine model.

机构信息

Henan Key Laboratory of Helicobacter pylori & Microbiota and Gastrointestinal Cancer, Marshall Medical Research Center, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Department of Gastroenterology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

出版信息

Front Cell Infect Microbiol. 2023 Aug 9;13:1196084. doi: 10.3389/fcimb.2023.1196084. eCollection 2023.

DOI:10.3389/fcimb.2023.1196084

PMID:37621875

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10445763/

Abstract

PURPOSE

To determine the role of Lactobacillus strains and their combinations in inhibiting the colonization of and gastric mucosa inflammation.

METHODS

Human gastric adenocarcinoma AGS cells were incubated with H. pylori and six probiotic strains ( NCFM, La-14, Lp-115, Lpc-37, Lr-32, and GG) and the adhesion ability of in different combinations was evaluated by fluorescence microscopy and urease activity assay. Male C57BL/6 mice were randomly divided into five groups (uninfected, , +NCFM, +Lp-115, and +NCFM+Lp-115) and treated with two lactobacilli strains (NCFM and Lp-115) for six weeks. colonization and tissue inflammation statuses were determined by rapid urease test, Hematoxylin-Eosin (HE) staining, immunohistochemistry, and qRT-PCR and ELISA.

RESULTS

NCFM, La-14, Lp-115, Lpc-37, Lr-32, and GG reduced adhesion and inflammation caused by infection in AGS cells and mice. Among all probiotics NCFM and , Lp-115 showed significant effects on the eradication and reduction of inflammation and . Compared with the infection group, the mRNA and protein expression levels of IL-8 and TNF-α in the six intervention groups were significantly reduced. The changes in the urease activity ( and ) for 1-7h in each group showed that NCFM, La-14, Lp-115, and GG effectively reduced the colonization of . We observed a higher ratio of lymphocyte and plasma cell infiltration into the lamina propria of the gastric mucosa and neutrophil infiltration in +NCFM+Lp-115 mice. The infiltration of inflammatory cells in lamina propria of the gastric mucosa was reduced in the +NCFM+Lp-115 group. Additionally, the expression of IFN-γ was decreased significantly in the NCFM and Lp-115 treated C57BL/6 mice.

CONCLUSIONS

NCFM and Lp-115 can reduce the adhesion of and inhibit the gastric inflammatory response caused by infection.

摘要

目的

确定乳杆菌菌株及其组合在抑制定植和胃黏膜炎症中的作用。

方法

将人胃腺癌 AGS 细胞与幽门螺杆菌和六种益生菌株（NCFM、La-14、Lp-115、Lpc-37、Lr-32 和 GG）孵育，并通过荧光显微镜和脲酶活性测定评估不同组合中幽门螺杆菌的粘附能力。雄性 C57BL/6 小鼠随机分为五组（未感染、感染、+NCFM、+Lp-115 和+NCFM+Lp-115），并接受两种乳杆菌菌株（NCFM 和 Lp-115）治疗六周。通过快速尿素酶试验、苏木精-伊红（HE）染色、免疫组织化学和 qRT-PCR 和 ELISA 测定幽门螺杆菌定植和组织炎症状态。

结果

NCFM、La-14、Lp-115、Lpc-37、Lr-32 和 GG 减少了 AGS 细胞和小鼠中幽门螺杆菌感染引起的粘附和炎症。在所有益生菌中，NCFM 和 Lp-115 对幽门螺杆菌的根除和炎症减轻有显著效果。与感染组相比，六个幽门螺杆菌干预组的 IL-8 和 TNF-αmRNA 和蛋白表达水平均显著降低。每组 1-7 小时的脲酶活性（和）变化表明，NCFM、La-14、Lp-115 和 GG 有效降低了幽门螺杆菌的定植。我们观察到胃黏膜固有层淋巴细胞和浆细胞浸润以及中性粒细胞浸润的比例增加，在+NCFM+Lp-115 小鼠中。+NCFM+Lp-115 组胃黏膜固有层炎症细胞浸润减少。此外，在 NCFM 和 Lp-115 处理的 C57BL/6 小鼠中，IFN-γ的表达显著降低。