Kawooya Abubaker, Emmanuel Eilu, Nakavuma Jesca, Kumar Sunil, Kafeero Hussein Mukasa, Mwambi Ddembe Bashir, Adeyemo Rasheed Omotayo, Adebayo Ismail Abiola, Akinola Saheed Adekunle
Department of Microbiology and Immunology, Kampala International University-Western Campus, Bushenyi, Uganda.
College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, P. O. Box 7062, Kampala, Uganda.
Data Brief. 2024 Mar 20;54:110350. doi: 10.1016/j.dib.2024.110350. eCollection 2024 Jun.
This article presents a dataset on bacterial community structure associated with Ready-to-eat (RTE) vegetable salads sold in Kampala City, Uganda. The Illumina Miseq sequencing of 16S rRNA gene amplicon unveiled the bacterial communities and generated a metagenomic library from RTE vegetable salads to understand the diversities and distribution. The metagenome contained a total of 23,805 sequences with 35,420 Taxonomic units (OTUs). Metagenome sequence information is obtainable at NCBI under the Bioproject assigned accession number PRJNA1064313. Taxonomic hits distribution from VSEARCH analysis at phylum level classification of NN-3 discovered predominantly Proteobacteria (65.34%) followed by Firmicutes (31.60%) and Bacteroidota (0.14%). Deinococcota (0.01%) and Planctomycetota (0.01%) were also detected. Also, VSEARCH-assisted analysis of NN-4 detected a higher prevalence of Firmicutes (65.68%) than Proteobacteria (33.25%), while Bacteroidota (0.04%) indicating the presence of contaminants of faecal sources.
本文展示了一组关于乌干达坎帕拉市销售的即食（RTE）蔬菜沙拉相关细菌群落结构的数据集。对16S rRNA基因扩增子进行的Illumina Miseq测序揭示了细菌群落，并从即食蔬菜沙拉中生成了一个宏基因组文库，以了解其多样性和分布情况。该宏基因组共包含23,805个序列和35,420个分类单元（OTU）。宏基因组序列信息可在NCBI上获取，其生物项目的登录号为PRJNA1064313。在对NN - 3进行门水平分类的VSEARCH分析中，分类命中分布显示主要为变形菌门（65.34%），其次是厚壁菌门（31.60%）和拟杆菌门（0.14%）。还检测到了异常球菌门（0.01%）和浮霉菌门（0.01%）。此外，对NN - 4的VSEARCH辅助分析检测到厚壁菌门的患病率（65.68%）高于变形菌门（33.25%），而拟杆菌门（0.04%）表明存在粪便源污染物。
