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用于野生型和过表达核因子Y亚基A7的转基因马铃薯植株之间差异表达基因比较分析的转录组数据集。

Transcriptome dataset for comparative analysis of differentially expressed genes between wild type and transgenic potato plants overexpressing Nuclear Factor Y subunit A7.

作者信息

Azeez Bimpe Suliyat, Kim Dool-Yi, Na Jong-Kuk

机构信息

Department of Agriculture and Industries, Graduate School, Kangwon National University, Chuncheon 24341, South Korea.

National Institute of Crop Science, RDA, Wanju 55365, South Korea.

出版信息

Data Brief. 2024 Mar 21;54:110349. doi: 10.1016/j.dib.2024.110349. eCollection 2024 Jun.

DOI:10.1016/j.dib.2024.110349
PMID:38586149
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10997943/
Abstract

Nuclear Factor Y (NF-Y) is divided into three different types of subunits, A, B, and C. NF-Ys play crucial roles in plants for controlling gene expression associated with various developmental processes and abiotic stresses, but it is mostly unknown the downstream genes regulated by NF-Ys in plant. One of the potato NF-Y genes, , increased potato's drought tolerance when overexpressed under the control of constitutive promoter. Therefore, it was of interest what genes are regulated by the increased expression level of . To investigate the downstream genes of , the transcriptome sequencing was carried out for four potato lines, including L 'Superior' as wild type (WT), empty vector control (VC), and two overexpressor lines (designated to #19 & #26). The RNA sequencing data was produced by the Illumina NovaSeq 6000 sequencing system. The number of total raw reads obtained from the RNA sequencing was 36.7 million for WT, 36.2 for VC, 29.3 for StNF-YA7 #19, and 29.5 million for #26, respectively. The length of total raw reads for each sample was between 5.92 Gb ( #19) and 7.42 Gb (WT), and after filtering raw quality reads, the total length was between 5.81 Gb ( #19) and 7.29 Gb (WT). Each filtered clear read set of four transcriptomes was mapped on the potato reference genome, SolTub_3.0, and the percentage of mapped reads ranged from 89.8 % (VC) to 90.3 % (WT). GC contents range between 43.01 % ( #19) and 42.44 % ( #26). Q20 quality score ranges between 98.63 % ( #26) and 98.74 % (VC).

摘要

核因子Y(NF-Y)分为三种不同类型的亚基,即A、B和C。NF-Y在植物中对控制与各种发育过程和非生物胁迫相关的基因表达起着关键作用,但在植物中由NF-Y调控的下游基因大多未知。马铃薯NF-Y基因之一,在组成型启动子的控制下过表达时提高了马铃薯的耐旱性。因此,受该基因表达水平增加调控的基因是什么很值得关注。为了研究该基因的下游基因,对四个马铃薯品系进行了转录组测序,包括作为野生型(WT)的“优等”L、空载体对照(VC)以及两个过表达株系(命名为#19和#26)。RNA测序数据由Illumina NovaSeq 6000测序系统产生。从RNA测序获得的原始 reads总数,WT为3670万,VC为3620万,StNF-YA7 #19为2930万,#26为2950万。每个样本的原始 reads总长度在5.92 Gb(#19)和7.42 Gb(WT)之间,在过滤原始质量 reads后,总长度在5.81 Gb(#19)和7.29 Gb(WT)之间。四个转录组的每个过滤后的清晰 reads集都映射到马铃薯参考基因组SolTub_3.0上,映射 reads的百分比范围从89.8%(VC)到90.3%(WT)。GC含量在43.01%(#19)和42.44%(#26)之间。Q20质量分数在98.63%(#26)和98.74%(VC)之间。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1566/10997943/58cd7a3603a2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1566/10997943/58cd7a3603a2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1566/10997943/58cd7a3603a2/gr1.jpg

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