Li Shigui, Zhang Ning, Zhu Xi, Ma Rui, Liu Shengyan, Wang Xiao, Yang Jiangwei, Si Huaijun
State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, China.
College of Agronomy, Gansu Agricultural University, Lanzhou, China.
Front Plant Sci. 2021 Oct 12;12:749688. doi: 10.3389/fpls.2021.749688. eCollection 2021.
The nuclear factor Y (NF-Y) family is comprised of transcription factors that have been implicated in multiple plant biological processes. However, little is known about this family in potato. In the present study, a total of 41 genes were identified in the potato genome. In addition, the phylogenetic, gene structure, motif, and chromosomal location of this family were analyzed. The tissue expression profiles based on RNA-seq data showed that 27 genes had tissue-specific expression, while the remaining 14 had low expression in all tissues. Publicly available transcriptomics data from various abiotic stresses revealed several stress-responsive genes, which were further verified quantitative real-time polymerase chain reaction experiments. Furthermore, the gene was highly induced by dehydration and drought treatments. StNF-YC9 protein was mainly localized in the nucleus and cytoplasmic membrane. Overexpressing StNF-YC9 potato lines (OxStNF-YC9) had significantly increased in root length and exhibited stronger stomatal closure in potato treated by polyethylene-glycol and abscisic acid. In addition, OxStNF-YC9 lines had higher photosynthetic rates and decreased water loss under short-term drought stress compared to wild-type plants. During long-term drought stress, OxStNF-YC9 lines had higher proline levels, lower malondialdehyde content, and increased activity of several antioxidant enzymes, including superoxide dismutase, catalase, and peroxidase. This study increased our understanding of the gene and suggested that played an important role in drought tolerance by increased the photosynthesis rate, antioxidant enzyme activity, and proline accumulation coupled to lowered malondialdehyde accumulation in potato.
核因子Y（NF-Y）家族由参与多种植物生物学过程的转录因子组成。然而，关于马铃薯中的这个家族我们知之甚少。在本研究中，在马铃薯基因组中总共鉴定出41个基因。此外，还分析了该家族的系统发育、基因结构、基序和染色体定位。基于RNA测序数据的组织表达谱显示，27个基因具有组织特异性表达，而其余14个在所有组织中表达较低。来自各种非生物胁迫的公开转录组学数据揭示了几个胁迫响应基因，这些基因通过定量实时聚合酶链反应实验得到进一步验证。此外，该基因在脱水和干旱处理下被高度诱导。StNF-YC9蛋白主要定位于细胞核和细胞质膜。过表达StNF-YC9的马铃薯株系（OxStNF-YC9）根长显著增加，并且在聚乙二醇和脱落酸处理的马铃薯中表现出更强的气孔关闭。此外，与野生型植株相比，OxStNF-YC9株系在短期干旱胁迫下具有更高的光合速率和更低的水分流失。在长期干旱胁迫期间，OxStNF-YC9株系具有更高的脯氨酸水平、更低的丙二醛含量以及几种抗氧化酶（包括超氧化物歧化酶、过氧化氢酶和过氧化物酶）活性的增加。本研究增进了我们对该基因的了解，并表明其通过提高马铃薯的光合速率、抗氧化酶活性和脯氨酸积累以及降低丙二醛积累，在耐旱性中发挥重要作用。
