College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China.
College of Public Health, Qingdao University, Qingdao, 266000, China.
Food Funct. 2024 May 7;15(9):4862-4873. doi: 10.1039/d4fo00962b.
Intestinal infections are strongly associated with infant mortality, and intestinal immunoglobulin A (IgA) is important to protect infants from intestinal infections after weaning. This study aims to screen probiotics that can promote the production of intestinal IgA after weaning and further explore their potential mechanisms of action. In this study, probiotics promoting intestinal IgA production were screened in weanling mouse models. The results showed that oral administration of () FL228.1 and () FL276.1 significantly enhanced IgA levels in the small intestine and upregulated the expression of a proliferation-inducing ligand (APRIL) and its upstream regulatory factor toll-like receptor 4 (TLR4). Furthermore, FL228.1 upregulated the relative abundance of , while FL276.1 increased the relative abundance of and decreased , further elevating intestinal IgA levels. In summary, FL228.1 and FL276.1 can induce IgA production in the intestinal tract of weanling mice by promoting intestinal APRIL expression and mediating changes in the gut microbiota, thus playing a significant role in enhancing local intestinal immunity in infants.
肠道感染与婴儿死亡率密切相关，而肠免疫球蛋白 A（IgA）对于婴儿在断奶后免受肠道感染非常重要。本研究旨在筛选能促进断奶后肠道 IgA 产生的益生菌，并进一步探索其潜在的作用机制。本研究在断奶小鼠模型中筛选了能促进肠道 IgA 产生的益生菌。结果表明，口服 () FL228.1 和 () FL276.1 能显著提高小肠中 IgA 的水平，并上调增殖诱导配体（APRIL）及其上游调节因子 Toll 样受体 4（TLR4）的表达。此外，() FL228.1 上调了 的相对丰度，而 () FL276.1 增加了 和减少了 的相对丰度，从而进一步提高了肠道 IgA 的水平。综上所述，() FL228.1 和 () FL276.1 可通过促进肠 APRIL 表达和调节肠道微生物群的变化来诱导断奶小鼠肠道 IgA 的产生，从而在增强婴儿局部肠道免疫方面发挥重要作用。
