锥虫可变表面糖蛋白羧基末端的快速加工
Rapid processing of the carboxyl terminus of a trypanosome variant surface glycoprotein.
作者信息
Bangs J D, Hereld D, Krakow J L, Hart G W, Englund P T
出版信息
Proc Natl Acad Sci U S A. 1985 May;82(10):3207-11. doi: 10.1073/pnas.82.10.3207.
Abstract
The variant surface glycoprotein of the parasite Trypanosoma brucei contains a glycolipid of unknown structure covalently attached to its COOH terminus. We have shown, by using metabolic labeling with [35S]methionine or [3H]myristic acid, precipitation with specific antibodies, and NaDodSO4/polyacrylamide gel electrophoresis, that this glycolipid is attached to the variant surface glycoprotein polypeptide within 1 min after its translation.
摘要
布氏锥虫寄生虫的可变表面糖蛋白含有一种结构未知的糖脂，该糖脂共价连接在其COOH末端。我们通过使用[35S]甲硫氨酸或[3H]肉豆蔻酸进行代谢标记、用特异性抗体沉淀以及NaDodSO4/聚丙烯酰胺凝胶电泳表明，这种糖脂在翻译后1分钟内就连接到可变表面糖蛋白多肽上。
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