Ferguson M A, Cross G A
J Biol Chem. 1984 Mar 10;259(5):3011-5.
A variant surface glycoprotein (VSG) of the parasitic protozoan Trypanosoma brucei was purified following the direct solubilization of trypanosomes in a boiling detergent solution. This material behaved as the amphiphilic membrane form of the glycoprotein previously described (Cardoso de Almeida, M. L., and Turner, M. J. (1983) Nature (Lond.) 302, 349-352). Analysis of this material showed that it contained ester-linked tetradecanoic (myristic) acid. After biosynthetic labeling of trypanosomes with [3H]myristic acid, it was shown that the release of the VSG coat from the parasite membrane, as the soluble form of the VSG, occurred concomitantly with the loss of myristic acid from the glycoprotein. The results suggest that VSG is attached to the parasite membrane via a covalently linked myristic acid-containing lipid moiety.
寄生原生动物布氏锥虫的一种变异表面糖蛋白（VSG），是通过将锥虫直接溶解于沸腾的去污剂溶液中后进行纯化的。该物质表现为先前描述的糖蛋白的两亲性膜形式（卡尔多索·德阿尔梅达，M. L.，和特纳，M. J.（1983年）《自然》（伦敦）302，349 - 352）。对该物质的分析表明，它含有酯连接的十四烷酸（肉豆蔻酸）。在用[³H]肉豆蔻酸对锥虫进行生物合成标记后，发现VSG外壳以VSG的可溶形式从寄生虫膜上释放，同时糖蛋白中的肉豆蔻酸也随之丢失。结果表明，VSG通过共价连接的含肉豆蔻酸的脂质部分附着于寄生虫膜上。
