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FTA 卡在兽医领域两种 RNA（猪瘟病毒和猪细小病毒）和两种 DNA 病毒（非洲猪瘟病毒和猪圆环病毒 1 型）检测中的应用。

Use of FTA card for the detection of two RNA (CSFV and SV-A) and two DNA viruses (ASFVand SuHV-1) of importance in veterinary medicine.

机构信息

Laboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Brasil.

出版信息

Braz J Microbiol. 2024 Jun;55(2):1961-1966. doi: 10.1007/s42770-024-01309-x. Epub 2024 Apr 9.

DOI:10.1007/s42770-024-01309-x
PMID:38589741
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11153393/
Abstract

The FTA card has emerged as a promising alternative for nucleic acid extraction. The FTA card is a filter paper impregnated with chemicals that preserve and stabilize the genetic material present in the sample, allowing for its storage and transport at room temperature. The aim of this study was to test the card for the detection of RNA and DNA nucleic acids. Two RNA viruses (Senecavirus A and classical swine fever virus) and two DNA viruses (African swine fever virus and suid alphaherpesvirus 1) were tested, and in all cases, there was a decrease in sensitivity. The methods exhibited good repeatability and demonstrated a rapid and practical use for sample transport and nucleic acid extraction.

摘要

FTA 卡已成为一种有前途的核酸提取替代方法。FTA 卡是一种用化学物质浸渍的滤纸，可保存和稳定样品中存在的遗传物质，使其能够在室温下储存和运输。本研究旨在测试该卡检测 RNA 和 DNA 核酸的能力。我们测试了两种 RNA 病毒（塞尼卡病毒 A 和经典猪瘟病毒）和两种 DNA 病毒（非洲猪瘟病毒和猪α疱疹病毒 1），在所有情况下，检测的灵敏度都有所下降。这些方法表现出良好的重复性，并且证明了它们在样本运输和核酸提取方面的快速实用用途。

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本文引用的文献

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FTA Cards as a Rapid Tool for Collection and Transport of Infective Samples: Experience with Foot-and-Mouth Disease Virus in Libya.FTA卡作为采集和运送感染性样本的快速工具：利比亚口蹄疫病毒的经验
Animals (Basel). 2022 Nov 18;12(22):3198. doi: 10.3390/ani12223198.
2
Usage of FTA Classic Cards for Safe Storage, Shipment, and Detection of Arboviruses.使用FTA经典卡片进行虫媒病毒的安全储存、运输和检测。
Microorganisms. 2022 Jul 18;10(7):1445. doi: 10.3390/microorganisms10071445.
3
Evaluation of Whatman FTA cards for the preservation of yellow fever virus RNA for use in molecular diagnostics.评价沃特曼 FTA 卡对黄热病病毒 RNA 的保存效果，以便于在分子诊断中使用。
PLoS Negl Trop Dis. 2022 Jun 15;16(6):e0010487. doi: 10.1371/journal.pntd.0010487. eCollection 2022 Jun.
4
Optimizing Release of Nucleic Acids of and from FTA Cards.优化从 FTA 卡中释放 和 的核酸。
Int J Mol Sci. 2021 Nov 29;22(23):12915. doi: 10.3390/ijms222312915.
5
Evaluation method for asymmetric uncertainty of quantitative polymerase chain reaction measurements of deoxyribonucleic acids with low copy number.低拷贝数脱氧核糖核酸定量聚合酶链反应测量的不对称不确定度评价方法。
Sci Rep. 2021 Jun 2;11(1):11550. doi: 10.1038/s41598-021-90959-0.
6
Performance characteristics of a quantitative PCR assay on repository stool specimens and smeared filter-paper cards.储存粪便标本和涂抹滤纸片的定量 PCR 检测的性能特征。
BMC Res Notes. 2020 Oct 30;13(1):500. doi: 10.1186/s13104-020-05340-7.
7
A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?FTA 卡®作为野外工作中病毒 RNA 保存工具的系统评价：它们安全有效吗？
Prev Vet Med. 2019 Nov 15;172:104772. doi: 10.1016/j.prevetmed.2019.104772. Epub 2019 Sep 10.
8
Consequences of measurement error in qPCR telomere data: A simulation study.qPCR 端粒数据测量误差的后果：一项模拟研究。
PLoS One. 2019 May 1;14(5):e0216118. doi: 10.1371/journal.pone.0216118. eCollection 2019.
9
Porcine reproductive and respiratory syndrome virus RNA detection in different matrices under typical storage conditions in the UK.在英国典型储存条件下不同基质中猪繁殖与呼吸综合征病毒 RNA 的检测。
Vet Rec. 2019 Jul 6;185(1):21. doi: 10.1136/vr.105312. Epub 2019 Apr 30.
10
FTA Cards for Preservation of Nucleic Acids for Molecular Assays: A Review on the Use of Cytologic/Tissue Samples.用于分子检测的核酸保存的 FTA 卡：细胞/组织样本应用综述。
Arch Pathol Lab Med. 2018 Mar;142(3):308-312. doi: 10.5858/arpa.2017-0303-RA.