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探索源自蝎子的 Css54 肽对白色念珠菌的治疗潜力。

Exploring the Therapeutic Potential of Scorpion-Derived Css54 Peptide Against Candida albicans.

机构信息

Department of Bioinformatics, Kongju National University, Kongju, 32588, Republic of Korea.

Department of Biomedical Science, Chosun University, Gwangju, 61452, Republic of Korea.

出版信息

J Microbiol. 2024 Feb;62(2):101-112. doi: 10.1007/s12275-024-00113-4. Epub 2024 Apr 8.

DOI:10.1007/s12275-024-00113-4
PMID:38589765
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11021323/
Abstract

Candida albicans (C. albicans) is one of the most common opportunistic fungi worldwide, which is associated with a high mortality rate. Despite treatment, C. albicans remains the leading cause of life-threatening invasive infections. Consequently, antimicrobial peptides (AMPs) are potential alternatives as antifungal agents with excellent antifungal activity. We previously reported that Css54, found in the venom of Centrurodies suffusus suffusus (C. s. suffusus) showed antibacterial activity against zoonotic bacteria. However, the antifungal activity of Css54 has not yet been elucidated. The objective of this study was to identify the antifungal activity of Css54 against C. albicans and analyze its mechanism. Css54 showed high antifungal activity against C. albicans. Css54 also inhibited biofilm formation in fluconazole-resistant fungi. The antifungal mechanism of action of Css54 was investigated using membrane-related assays, including the membrane depolarization assay and analysis of the membrane integrity of C. albicans after treatment with Css54. Css54 induced reactive oxygen species (ROS) production in C. albicans, which affected its antifungal activity. Our results indicate that Css54 causes membrane damage in C. albicans, highlighting its value as a potential therapeutic agent against C. albicans infection.

摘要

白色念珠菌（Candida albicans，C. albicans）是全球最常见的机会性真菌之一，与高死亡率相关。尽管进行了治疗，C. albicans 仍然是导致危及生命的侵袭性感染的主要原因。因此，抗菌肽（antimicrobial peptides，AMPs）作为具有优异抗真菌活性的抗真菌药物具有很大的应用潜力。我们之前的研究报告称，Centruroides suffusus suffusus（C. s. suffusus）毒液中的 Css54 具有抗动物源细菌的抗菌活性。然而，Css54 的抗真菌活性尚未阐明。本研究旨在确定 Css54 对白色念珠菌的抗真菌活性，并分析其机制。Css54 对白色念珠菌表现出很高的抗真菌活性。Css54 还抑制了氟康唑耐药真菌的生物膜形成。通过膜相关测定（包括膜去极化测定和 Css54 处理后白色念珠菌膜完整性分析）研究了 Css54 的抗真菌作用机制。Css54 诱导了白色念珠菌中活性氧（reactive oxygen species，ROS）的产生，从而影响了其抗真菌活性。我们的结果表明 Css54 导致了白色念珠菌的膜损伤，这突出了其作为抗白色念珠菌感染的潜在治疗剂的价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a0/11021323/e93e4d82c236/12275_2024_113_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a0/11021323/779adab0fd37/12275_2024_113_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a0/11021323/f548abe77b0d/12275_2024_113_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a0/11021323/3d2dc184a155/12275_2024_113_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a0/11021323/4083ad288c06/12275_2024_113_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a0/11021323/a853b5e2b021/12275_2024_113_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a0/11021323/e93e4d82c236/12275_2024_113_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a0/11021323/779adab0fd37/12275_2024_113_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a0/11021323/f548abe77b0d/12275_2024_113_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a0/11021323/3d2dc184a155/12275_2024_113_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a0/11021323/4083ad288c06/12275_2024_113_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a0/11021323/a853b5e2b021/12275_2024_113_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a0/11021323/e93e4d82c236/12275_2024_113_Fig6_HTML.jpg

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