Huang Jinli, Wang Xingzhi, Li Qiuhong, Zhang Panpan, Jing Zenghui, Zhang Juan, Su Hui, Sun Xin
Department of Pediatrics, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
Department of Geriatrics, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
Int J Microbiol. 2024 Mar 31;2024:7172386. doi: 10.1155/2024/7172386. eCollection 2024.
Atopic dermatitis is one of the most common dermatologic problems, especially in children. Given the ability of symbiotic microorganisms in modulating the immune system, probiotics administration has been studied in previous research in the management of atopic dermatitis. However, there are conflicting results between studies. In this study, we aimed to assess the effectiveness of mixed probiotics as a treatment option for atopic dermatitis induced by ovalbumin. BALB/c juvenile mice were classified and divided into the ovalbumin group, mixed probiotic group (ovalbumin + LK), and control group. Except for the control group, all mice were sensitized with ovalbumin to establish a model of atopic dermatitis. The mixed probiotics were given by gavage for 14 days. Mice body weight, skin lesions, skin inflammation, ovalbumin-specific Ig, the number of Treg and CD103DC, and the expression level of PD-1/PD-L1 were examined. The results showed that mixed probiotics can improve body weight and alleviate skin symptoms. Mixed probiotics reduced serum Th2 inflammatory factors, eosinophils, mast cell degranulation, mast cell count, and the expression of ovalbumin-specific immunoglobulin E/G1 and increased the anti-inflammatory cytokine interleukin-10, Treg cells, CD103DC cells, and the expression level of PD-1/PD-L1. These findings suggest that mixed probiotics could be a viable treatment option for atopic dermatitis and provide insight into the underlying mechanisms involved.
特应性皮炎是最常见的皮肤问题之一，尤其是在儿童中。鉴于共生微生物具有调节免疫系统的能力，先前的研究已对益生菌给药在特应性皮炎管理中的作用进行了研究。然而，各研究结果存在矛盾。在本研究中，我们旨在评估混合益生菌作为卵清蛋白诱导的特应性皮炎治疗选择的有效性。将BALB/c幼年小鼠分类并分为卵清蛋白组、混合益生菌组（卵清蛋白+LK）和对照组。除对照组外，所有小鼠均用卵清蛋白致敏以建立特应性皮炎模型。通过灌胃给予混合益生菌14天。检测小鼠体重、皮肤病变、皮肤炎症、卵清蛋白特异性Ig、调节性T细胞（Treg）和CD103树突状细胞（DC）数量以及程序性死亡受体1（PD-1）/程序性死亡配体1（PD-L1）的表达水平。结果表明，混合益生菌可改善体重并减轻皮肤症状。混合益生菌降低了血清Th2炎症因子、嗜酸性粒细胞、肥大细胞脱颗粒、肥大细胞计数以及卵清蛋白特异性免疫球蛋白E/G1的表达，并增加了抗炎细胞因子白细胞介素-10、Treg细胞、CD103DC细胞以及PD-1/PD-L1的表达水平。这些发现表明，混合益生菌可能是特应性皮炎的一种可行治疗选择，并为其中涉及的潜在机制提供了见解。
