Askari Hamed, Soleimanian-Zad Sabihe, Kadivar Mahdi, Shahbazi Samira
Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Research Institute for Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Heliyon. 2024 Mar 24;10(7):e28349. doi: 10.1016/j.heliyon.2024.e28349. eCollection 2024 Apr 15.
Creating novel sources of a microbial strain using induced mutation can increase enzyme production for industrial use. According to this, we have developed a mutant strain of by Co gamma irradiation. mutants were isolated from an optimum dose of 250 Gy. The qualitative and quantitative screening were used for evaluating their enzyme production and the DNA barcoding method was used to identify the best mutant isolates. The highest cellulase (exo-glucanase, endoglucanase, β-glucosidase, and total cellulase) and xylanase activities were observed in superior mutant isolates of NAS107-M44 and NAS107-M82, which is approximately 1.6-2.5 times higher than its parent strain, respectively. The electrophoretic pattern of proteins showed that the exo-glucanase I, endo-glucanase III, and the xylanase I enzymes hydrolyzed the corn bran, synergistically. Overall, gamma irradiation-induced mutation could be an expedient technique to access such superior mutants for the bioconversion of corn bran wastes.
利用诱导突变创造微生物菌株的新来源可以提高工业用酶的产量。据此，我们通过钴γ射线辐照开发了一种 的突变菌株。从250 Gy的最佳剂量中分离出 突变体。采用定性和定量筛选来评估它们的酶产量，并使用DNA条形码方法来鉴定最佳的 突变体分离株。在 NAS107-M44和 NAS107-M82的优良突变体分离株中观察到最高的纤维素酶（外切葡聚糖酶、内切葡聚糖酶、β-葡萄糖苷酶和总纤维素酶）和木聚糖酶活性，分别比其亲本菌株高约1.6 - 2.5倍。蛋白质电泳图谱表明，外切葡聚糖酶I、内切葡聚糖酶III和木聚糖酶I协同水解玉米麸皮。总体而言，γ射线辐照诱导的突变可能是一种便捷的技术，可用于获取此类优良突变体以进行玉米麸皮废物的生物转化。
