State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
Int J Biol Macromol. 2020 Jun 1;152:288-294. doi: 10.1016/j.ijbiomac.2020.02.256. Epub 2020 Feb 24.
Influence of water content on the expression of lignocellulolytic enzymes by Phanerochaete chrysosporium remains unclear. This work compares the enzyme production profiles of P. chrysosporium during solid-state and submerged fermentation. There were 110 and 64 extracellular carbohydrate-active enzymes identified in solid-state and submerged fermentation respectively, among which 57 enzymes were common to both of the secretomes. P. chrysosporium secreted more cellulases (especially lytic polysaccharide monooxygenase) and hemicellulases during solid-state fermentation while the proportion of enzyme containing carbohydrate-binding module was higher for submerged fermentation. Although its activities were weaker, the enzyme cocktail from submerged fermentation was surprisingly more effective in hydrolysis at low substrate loading. This advantage of enzymes from submerged fermentation was mainly attributed to carbohydrate-binding module because more xylanases bound with substrate at the beginning of hydrolysis. These results reveal the influence of fermentation conditions on enzyme produced by P. chrysosporium for the first time and show the importance of carbohydrate-binding module in the hydrolysis process of lignocellulose.
水分含量对黄孢原毛平革菌木质纤维素酶表达的影响尚不清楚。本工作比较了黄孢原毛平革菌固态发酵和液态发酵过程中的酶生产曲线。分别在固态发酵和液态发酵中鉴定到 110 种和 64 种胞外碳水化合物活性酶，其中 57 种酶存在于两种分泌组中。黄孢原毛平革菌在固态发酵过程中分泌更多的纤维素酶（特别是裂解多糖单加氧酶）和半纤维素酶，而在液态发酵中含碳水化合物结合模块的酶的比例更高。尽管其活性较弱，但来自液态发酵的酶混合物在低底物负荷下水解时效果惊人地更好。液态发酵酶的这种优势主要归因于碳水化合物结合模块，因为更多的木聚糖酶在水解开始时与底物结合。这些结果首次揭示了发酵条件对黄孢原毛平革菌产生的酶的影响，并表明碳水化合物结合模块在木质纤维素水解过程中的重要性。
