Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
Université Paris-Saclay, INRAE, AgroParisTech, UMR BIOGER, 78850, Thiverval-Grignon, France.
Int J Syst Evol Microbiol. 2020 Nov;70(11):5665-5670. doi: 10.1099/ijsem.0.004457. Epub 2020 Sep 14.
Two strains (DMKU-GTCP10-8 and CLIB 1740) representing a novel anamorphic yeast species were isolated from a grease sample collected from a grease trap in Thailand and from an unidentified fungus collected in French Guiana, respectively. On the basis of phylogenetic analysis based on the combined D1D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, CBS 707 was the closely related species with 12.8 % sequence divergence (70 nucleotide substitutions and three gaps in 571 nucleotides) and 28.1 % sequence divergence (93 nucleotide substitutions and 90 gaps in 651 nucleotides) in the D1/D2 domain of the LSU rRNA gene and the ITS region, respectively. Phylogenetic analysis based on the concatenated sequences of the five genes including the small subunit rRNA gene, the D1D2 domain of the LSU rRNA gene, the ITS region, translation elongation factor-1 alpha () and RNA polymerase II subunit 2 () genes confirmed that the two strains (DMKU-GTCP10-8 and CLIB 1740) were well-separated from other described yeast genera in Saccharomycetaceae. Hence, gen. nov., sp. nov. is proposed to accommodate these two strains as members of the family Saccharomycetaceae. The holotype is DMKUGTCP10-8 (extype strain TBRC 12159PYCC 8490; MycoBank number 835044).
两株（DMKU-GTCP10-8 和 CLIB 1740）分别代表了从泰国油脂收集器中分离的一种新型无性酵母种和从法属圭亚那分离的一种未鉴定真菌的新种。基于 D1D2 大亚基（ LSU ） rRNA 基因和内部转录间隔区（ ITS ）区域的联合序列分析， CBS 707 是最接近的种，其 LSU rRNA 基因的 D1/D2 结构域和 ITS 区分别具有 12.8%的序列差异（571 个核苷酸中有 70 个核苷酸取代和 3 个空位）和 28.1%的序列差异（651 个核苷酸中有 93 个核苷酸取代和 90 个空位）。基于包括小亚基 rRNA 基因、 LSU rRNA 基因的 D1D2 结构域、 ITS 区、延伸因子-1α（ EF-1α ）和 RNA 聚合酶 II 亚基 2 （ RPB2 ）基因的五个基因的串联序列的系统发育分析证实，这两个菌株（DMKU-GTCP10-8 和 CLIB 1740）与 Saccharomycetaceae 科中描述的其他酵母属分离良好。因此，提议建立新属，新种，以容纳这两个菌株作为 Saccharomycetaceae 科的成员。模式种为 DMKUGTCP10-8（extype 菌株 TBRC 12159PYCC 8490；MycoBank 编号 835044）。
